Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing

doi:10.1021/acssensars.7b00495

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
	Ren, Xiang 3; Yan, Junrong 1; Wu, Dan 3; Wei, Qin 3; Wan, Yakun 2
刊名	ACS SENSORS
	2017-09
卷号	2 期号:9 页码:1267-1271
关键词	nanobody apolipoprotein E point-of-care testing colorimetric immunosensor
ISSN号	2379-3694
DOI	10.1021/acssensars.7b00495
文献子类	Article
英文摘要	Alzheimer's disease (AD) biomarkers can reflect the neurochemical indicators used to estimate the risk in clinical nephrology. Apolipoprotein E (ApoE) is an early biomarker for AD in clinical diagnosis. In this research, through bactrian camel immunization, lymphocyte isolation, RNA extraction, and library construction, ApoE-specific Nbs with high affinity were successfully separated from an immune phage display nanobody library. Herein, a colorimetric immunosensor was developed for the point-of-care testing of ApoE by layer-by-layer nanoassembly techniques and novel nanobodies (Nbs). Using highly oriented Nbs as the capture and detection antibodies, an on-site immunosensor was developed by detecting the mean gray value of fade color due to the glutaraldehydeP3-aminopropyltrimethoxysilane oxidation by H2O2. The detection limit of AopE is 0.42 pg/mL, and the clinical analysis achieves a good performance. The novel easily operated immunosensor may have potential application in the clinical diagnosis and real-time monitoring for AD.
资助项目	National Key Scientific Instrument and Equipment Development Project of China[21627809] ; National Natural Science Foundation of China[21375047] ; National Natural Science Foundation of China[21575137] ; National Natural Science Foundation of China[21575050] ; National Natural Science Foundation of China[21601064] ; Special Foundation for Taishan Scholar Professorship of Shandong Province[ts20130937] ; Science and Technology Development Plan of Shandong Province[2014GSF120004] ; Graduate Innovation Foundation of UJN[YCXB15004]
WOS关键词	SINGLE-DOMAIN ANTIBODIES ; ANTIGEN ; GRAPHENE ; ELECTROCHEMILUMINESCENCE ; IMMUNOASSAY ; DISEASE ; PROBE
WOS研究方向	Chemistry ; Science & Technology - Other Topics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000411853000004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272493]
专题	药理学第一研究室
通讯作者	Wei, Qin; Wan, Yakun
作者单位	1.Southeast Univ, Inst Life Sci, Nanjing 210000, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 3.Univ Jinan, Univ Shandong, Key Lab Interfacial React & Sensing Anal, Sch Chem & Chem Engn, Jinan 250022, Shandong, Peoples R China;
推荐引用方式 GB/T 7714	Ren, Xiang,Yan, Junrong,Wu, Dan,et al. Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing[J]. ACS SENSORS,2017,2(9):1267-1271.
APA	Ren, Xiang,Yan, Junrong,Wu, Dan,Wei, Qin,&Wan, Yakun.(2017).Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing.ACS SENSORS,2(9),1267-1271.
MLA	Ren, Xiang,et al."Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing".ACS SENSORS 2.9(2017):1267-1271.