Determination of levosimendan and its main metabolites in  human plasma with HPLC-MS/MS method

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海药物代谢研究中心

	Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method
	LI Shaorong 2; CHEN Xiaoyan1 ; ZHANG Yifan1 ; LI Guoxin 3; JIANG Chunmei 3; ZHONG Dafang2
刊名	Acta Pharmaceutica Sinica
	2008
卷号	43 期号:10 页码:1053-1059
关键词	OR-1855 OR-1896 levosimendan 0R-1855 0R-1896 LC-MS/MS pharmacokinetics
ISSN号	0513-4870
其他题名	HPLC-MS／MS法测定人血浆中的左西孟旦及其主要代谢物
文献子类	Article
英文摘要	This paper is aimed to develop rapid, sensitive and convenient HPLC-MS/MS methods for the quantification of levosimendan and its metabolites OR-1855 and OR-1896 in human plasma. According to the different natures of the compounds, two sets of liquid chromatography and ionization modes were used for determination the concentration of levosimendan and its metabolites OR-1855 and OR-1896 in human plasma, separately. Following protein precipitation with methanol, the levosimendan and internal standard （rosuvastatin） were separated on a Capcell MG III C_(18) column （35 mm 2.0 mm ID, 3 um） with the mobile phase consisted of methanol-15 mmol · L~(-1) ammonium acetate-formic acid （55：45：0. 02, v/v/v）. A tandem mass spectrometer equipped with electrospray ionization source was used as the detector and operated in the negative ion mode. Its metabolites 0R-1855, 0R-1896 and internal standard doxofylline were extracted from plasma by liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a Zorbax Extend C_(18) column（ 150 mm 4.6 mm ID, 5 um）with the mobile phase consisted of methanol-15 mmol · L~(-1) ammonium acetate-formic acid （65 ： 35 ： 0. 1, v/v/v）. A tandem mass spectrometer operated at the positive equipped with electrospray ionization source was used as the detector and ion mode. The linear concentration ranges of the calibration curves for levosimendan and OR-1855 and OR-1896 were 0. 10 -50. 0 ng · mL~(-1) , 0. 20 - 100 ng · mL~(-1), 0. 20 - 100 ng· mL~(-1), respectively. The lower limits of quantification of levosimendan and 0R-1855 and OR-1896 were 0. 10 ng· mL~(-1) , 0. 20 ng· mL~(-1) , 0. 20 ng· mL~(-1) , respectively. The methods proved to be sensitive, simple and rapid, and suitable for the pharmaeokinetic study of levosimendan injection.
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:3400844
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268097]
专题	上海药物代谢研究中心
通讯作者	ZHONG Dafang
作者单位	1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; 3.Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110034, China.
推荐引用方式 GB/T 7714	LI Shaorong,CHEN Xiaoyan,ZHANG Yifan,et al. Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method[J]. Acta Pharmaceutica Sinica,2008,43(10):1053-1059.
APA	LI Shaorong,CHEN Xiaoyan,ZHANG Yifan,LI Guoxin,JIANG Chunmei,&ZHONG Dafang.(2008).Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method.Acta Pharmaceutica Sinica,43(10),1053-1059.
MLA	LI Shaorong,et al."Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method".Acta Pharmaceutica Sinica 43.10(2008):1053-1059.