Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method | |
LI Shaorong2; CHEN Xiaoyan1![]() ![]() ![]() | |
刊名 | Acta Pharmaceutica Sinica
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2008 | |
卷号 | 43期号:10页码:1053-1059 |
关键词 | OR-1855 OR-1896 levosimendan 0R-1855 0R-1896 LC-MS/MS pharmacokinetics |
ISSN号 | 0513-4870 |
其他题名 | HPLC-MS/MS法测定人血浆中的左西孟旦及其主要代谢物 |
文献子类 | Article |
英文摘要 | This paper is aimed to develop rapid, sensitive and convenient HPLC-MS/MS methods for the quantification of levosimendan and its metabolites OR-1855 and OR-1896 in human plasma. According to the different natures of the compounds, two sets of liquid chromatography and ionization modes were used for determination the concentration of levosimendan and its metabolites OR-1855 and OR-1896 in human plasma, separately. Following protein precipitation with methanol, the levosimendan and internal standard (rosuvastatin) were separated on a Capcell MG III C_(18) column (35 mm *2.0 mm ID, 3 um) with the mobile phase consisted of methanol-15 mmol · L~(-1) ammonium acetate-formic acid (55:45:0. 02, v/v/v). A tandem mass spectrometer equipped with electrospray ionization source was used as the detector and operated in the negative ion mode. Its metabolites 0R-1855, 0R-1896 and internal standard doxofylline were extracted from plasma by liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a Zorbax Extend C_(18) column( 150 mm *4.6 mm ID, 5 um)with the mobile phase consisted of methanol-15 mmol · L~(-1) ammonium acetate-formic acid (65 : 35 : 0. 1, v/v/v). A tandem mass spectrometer operated at the positive equipped with electrospray ionization source was used as the detector and ion mode. The linear concentration ranges of the calibration curves for levosimendan and OR-1855 and OR-1896 were 0. 10 -50. 0 ng · mL~(-1) , 0. 20 - 100 ng · mL~(-1), 0. 20 - 100 ng· mL~(-1), respectively. The lower limits of quantification of levosimendan and 0R-1855 and OR-1896 were 0. 10 ng· mL~(-1) , 0. 20 ng· mL~(-1) , 0. 20 ng· mL~(-1) , respectively. The methods proved to be sensitive, simple and rapid, and suitable for the pharmaeokinetic study of levosimendan injection. |
WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
语种 | 中文 |
CSCD记录号 | CSCD:3400844 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/268097] ![]() |
专题 | 上海药物代谢研究中心 |
通讯作者 | ZHONG Dafang |
作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; 3.Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110034, China. |
推荐引用方式 GB/T 7714 | LI Shaorong,CHEN Xiaoyan,ZHANG Yifan,et al. Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method[J]. Acta Pharmaceutica Sinica,2008,43(10):1053-1059. |
APA | LI Shaorong,CHEN Xiaoyan,ZHANG Yifan,LI Guoxin,JIANG Chunmei,&ZHONG Dafang.(2008).Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method.Acta Pharmaceutica Sinica,43(10),1053-1059. |
MLA | LI Shaorong,et al."Determination of levosimendan and its main metabolites in human plasma with HPLC-MS/MS method".Acta Pharmaceutica Sinica 43.10(2008):1053-1059. |
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