Applications of Liquid Chromatography-Tandem Mass Spectrometry to Detection and Characterization of Reactive Metabolites

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	Applications of Liquid Chromatography-Tandem Mass Spectrometry to Detection and Characterization of Reactive Metabolites
	Xie Cen; Zhong Dafang; Chen Xiaoyan
刊名	Journal of Chinese Mass Spectrometry Society
	2011
卷号	32 期号:1 页码:1-12
关键词	liquid chromatography-tandem mass spectrometry(LC-MS/MS) reactive metabolites trapping agents
ISSN号	1004-2997
其他题名	液相色谱-串联质谱法检测反应性代谢物
文献子类	Article
英文摘要	A number of therapeutical drugs were reported to undergo metabolic activation by drug-metabolizing enzymes. The bioactivation forms reactive metabolite(s), which readily covalently bind to macromolecules, such as proteins and DNA, and then lead to toxicities. In recent years, screening drug candidates for their tendency to generate reactive metabolites during drug discovery and development process and as well monitoring the bioactivation for post-marketing drugs have become increasingly important. Most reactive metabolites are electrophilic in nature and can react with nucleophiles. In vitro microsomal incubations, small nucleophilic molecules, such as glutathione, cyanide and amines are generally used to trap reactive metabolites. Structural elucidation of these stable adducts are conducted by liquid chromatography-tandem mass spectrometry. In this review, different mass spectrometers including triple quadrupole, ion trap, quadrupole-linear ion trap, and high-resolution mass spectrometer, employed for assessing reactive metabolites are described. The recent advances of different techniques and approaches are also discussed
资助项目	国家自然科学基金项目[00000000]
WOS研究方向	Chemistry (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:4150408
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267892]
专题	上海药物代谢研究中心
通讯作者	Chen Xiaoyan
作者单位	Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	Xie Cen,Zhong Dafang,Chen Xiaoyan. Applications of Liquid Chromatography-Tandem Mass Spectrometry to Detection and Characterization of Reactive Metabolites[J]. Journal of Chinese Mass Spectrometry Society,2011,32(1):1-12.
APA	Xie Cen,Zhong Dafang,&Chen Xiaoyan.(2011).Applications of Liquid Chromatography-Tandem Mass Spectrometry to Detection and Characterization of Reactive Metabolites.Journal of Chinese Mass Spectrometry Society,32(1),1-12.
MLA	Xie Cen,et al."Applications of Liquid Chromatography-Tandem Mass Spectrometry to Detection and Characterization of Reactive Metabolites".Journal of Chinese Mass Spectrometry Society 32.1(2011):1-12.