Metabolites of injected chlorogenic acid in rats

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	Metabolites of injected chlorogenic acid in rats
	Xie, Cen; Zhong, Da-Fang; Chen, Xiao-Yan
	2011-01
卷号	46
期号	1
页码	88-95
英文摘要	Chlorogenic acid (5-CQA) is one of the major components in some Chinese herbal injections. However, the metabolism of 5-CQA in rats after intravenous injection has not been determined. An ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) method was applied to identify the metabolites in bile, urine, feces and plasma after a single intravenous administration of 10 mg x kg(-1) 5-CQA to rats. Using MSE and mass defect filter techniques, a total of 35 metabolites were detected in bile, urine, feces and plasma. The predominant metabolites in bile were glutathione conjugates of O-methyl-5-CQA, accounting for approximately 80% of the metabolites excreted in bile. The major components in urine were parent drug, O-methyl-5-CQA, hydrolyzed metabolites and glucuronide conjugates. The major components in feces were O-methyl-5-CQA and its cysteine conjugates. The major component in plasma was the parent drug. The urinary and fecal excretion pathways were equally important to 5-CQA in rats. These results demonstrate that 5-CQA undergoes extensively metabolism in rats and are highly reactive to nucleophiles such as GSH. This finding indicates that attention should be paid on the injections containing 5-CQA, which may covalently bind to proteins, leading to allergenic drug reactions.
会议录	Yao xue xue bao = Acta pharmaceutica Sinica
文献子类	Meeting Abstract;Article
语种	中文
内容类型	会议论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266885]
专题	上海药物代谢研究中心
作者单位	Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
推荐引用方式 GB/T 7714	Xie, Cen,Zhong, Da-Fang,Chen, Xiao-Yan. Metabolites of injected chlorogenic acid in rats[C]. 见:.