Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells

doi:10.1016/j.celrep.2018.08.069

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	Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells
	Shiota, Hitoshi 9; Barral, Sophie 9; Buchou, Thierry 9; Tan, Minjia10 ; Coute, Yohann 2; Charbonnier, Guillaume 3; Reynoird, Nicolas 9; Boussouar, Faycal 9; Gerard, Matthieu 4; Zhu, Mingrui 10
刊名	CELL REPORTS
	2018-09-25
卷号	24 期号:13 页码:3477-+
ISSN号	2211-1247
DOI	10.1016/j.celrep.2018.08.069
文献子类	Article
英文摘要	Nuclear protein in testis (Nut) is a universal oncogenic driver in the highly aggressive NUT midline carcinoma, whose physiological function in male germ cells has been unclear. Here we show that expression of Nut is normally restricted to post-meiotic spermatogenic cells, where its presence triggers p300-dependent genome-wide histone H4 hyperacetylation, which is essential for the completion of histone-to-protamine exchange. Accordingly, the inactivation of Nut induces male sterility with spermatogenesis arrest at the histone-removal stage. Nut uses p300 and/or CBP to enhance acetylation of H4 at both K5 and K8, providing binding sites for the first bromodomain of Brdt, the testis-specific member of the BET family, which subsequently mediates genome-wide histone removal. Altogether, our data reveal the detailed molecular basis of the global histone hyperacetylation wave, which occurs before the final compaction of the male genome.
资助项目	Foundation pour la Recherche Medicale (FRM)[00000000] ; ANR Episperm3 program[00000000] ; INCa libre program[RPT13001CCA] ; Universite Grenoble Alpes[ANR-15-IDEX-02] ; Fondation ARC "Canc'air" project[RAC16042CLA] ; Plan Cancer[CH7-INS15B66] ; Plan Cancer[ASC16012CSA] ; Worldwide Cancer Research foundation grant[16-0280] ; European Commission[FP7-PEOPLE-2011-ITN] ; European Commission[PITN-GA-289880] ; La Ligue Nationale Contre Le Cancer[00000000] ; FRM fellowship[00000000] ; Inserm, GIS IBiSA, and Aix-Marseille Universite[ANR-10-INBS-0009-10] ; French National Research Agency ProFi grant[ANR-10-INBS-08-01] ; Wellcome Trust Cancer Development Fellowship[095751/Z/11/Z]
WOS关键词	MIDLINE CARCINOMA ; NUCLEOSOME EVICTION ; ACETYLATION ; BROMODOMAIN ; TRANSCRIPTION ; BRDT ; SPERMATOGENESIS ; DIFFERENTIATION ; PURIFICATION ; TRANSITION
WOS研究方向	Cell Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000445524700012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279571]
专题	化学蛋白质组学研究中心
通讯作者	Khochbin, Saadi
作者单位	1.EMBL Grenoble, BP 181,71 Ave Martyrs, F-38042 Grenoble 9, France; 2.Univ Grenoble Alpes, CEA, Inserm U1038, CEA,BIG BGE, F-38000 Grenoble, France; 3.Aix Marseille Univ, Inserm U1090, TGML, Platform IbiSA,TAGC, Marseille, France; 4.CEA, iBiTec S, F-91191 Gif Sur Yvette, France; 5.Struct Genom Consortium, Old Rd Campus Res Bldg,Roosevelt Dr, Oxford OX3 7DQ, England; 6.Ludwig Inst Canc Res, Old Rd Campus Res Bldg,Roosevelt Dr, Oxford OX3 7DQ, England; 7.Univ Leicester, Dept Mol & Cell Biol, Leicester Inst Struct & Chem Biol, Lancaster Rd, Leicester LE1 7RH, Leics, England; 8.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA 9.Univ Grenoble Alpes, Inst Adv Biosci, Inserm U1209, CNRS UMR 5309, F-38700 Grenoble, France; 10.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Shiota, Hitoshi,Barral, Sophie,Buchou, Thierry,et al. Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells[J]. CELL REPORTS,2018,24(13):3477-+.
APA	Shiota, Hitoshi.,Barral, Sophie.,Buchou, Thierry.,Tan, Minjia.,Coute, Yohann.,...&Khochbin, Saadi.(2018).Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells.CELL REPORTS,24(13),3477-+.
MLA	Shiota, Hitoshi,et al."Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells".CELL REPORTS 24.13(2018):3477-+.