ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299
	Li, Kui 1,2; Zhu, Zhi-Chuan 2; Liu, Yong-Jie 2; Liu, Ji-Wei 2; Wang, Hong-Tao 2; Xiong, Zhi-Qi 2; Shen, Xu1 ; Hu, Ze-Lan 2; Zheng, Jing 2
刊名	INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
	2013
卷号	6 期号:11 页码:2460-2467
关键词	ZFX non-small cell lung carcinoma growth migration senescence
ISSN号	1936-2625
文献子类	Article
英文摘要	ZFX (zinc finger transcription factor, X chromosome-linked) contributes to the maintenance of different types of stem cells and the progression of various cancers. We have previously reported that ZFX knockdown inhibits proliferation of glioma in vitro and in vivo. Since overexpression of ZFX in lung cancer tissue correlates with lymph node metastasis, we hypothesized that ZFX may play a role in lung cancer. In this study, we identified ZFX as a promoter of lung cancer growth and migration in a NSCLC (non-small cell lung carcinoma) cell line H1299. ZFX knockdown caused proliferation inhibition determined by MTT assay and colony formation assay, G0/G1 arrest of cell cycle and slightly increased proportion of apoptotic cells assessed by flow cytometry assay, decreased population of migrating cells showed by wound-healing assay, increased cell senescence evidenced by senescence-associated beta-galactosidase staining. ZFX knockdown also led to decreased proportion of tumor bearing mice and reduced mean tumor volume in a subcutaneous tumor model. In addition, western blot showed that ZFX knockdown down regulated a set of proteins involved in proliferation, survival and motility. Altogether, these results suggest that ZFX may be a potential therapeutic target for NSCLC.
资助项目	China Post-doctoral Science Special Foundation[2010-03259] ; Shanghai Committee of Science and Technology[11DZ2260600]
WOS关键词	SELF-RENEWAL ; CANCER ; PROLIFERATION ; APOPTOSIS ; ATM ; GENE
WOS研究方向	Oncology ; Pathology
语种	英语
出版者	E-CENTURY PUBLISHING CORP
WOS记录号	WOS:000328889300021
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277773]
专题	药理学第三研究室
通讯作者	Zheng, Jing
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.E China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai 200237, Peoples R China;
推荐引用方式 GB/T 7714	Li, Kui,Zhu, Zhi-Chuan,Liu, Yong-Jie,et al. ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2013,6(11):2460-2467.
APA	Li, Kui.,Zhu, Zhi-Chuan.,Liu, Yong-Jie.,Liu, Ji-Wei.,Wang, Hong-Tao.,...&Zheng, Jing.(2013).ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,6(11),2460-2467.
MLA	Li, Kui,et al."ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 6.11(2013):2460-2467.