Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B

doi:10.1101/gad.220095.113

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	Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B
	Montellier, Emilie 2; Boussouar, Faycal 2; Rousseaux, Sophie 2; Zhang, Kai 4; Buchou, Thierry 2; Fenaille, Francois 3; Shiota, Hitoshi 2; Debernardi, Alexandra 2; Hery, Patrick 12; Curtet, Sandrine 2
刊名	GENES & DEVELOPMENT
	2013-08-01
卷号	27 期号:15 页码:1680-1692
关键词	H2AZ BRDT male contraception reprogramming male infertility sex chromosome inactivation histone eviction
ISSN号	0890-9369
DOI	10.1101/gad.220095.113
文献子类	Article
英文摘要	The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified, we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B reassembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.
资助项目	ANR EpiSperm2 grant[00000000] ; INCa[00000000] ; "ARC libre'' funds[00000000] ; MTR bourse flechee[00000000] ; ARC fellowship[00000000] ; European Commission[00000000] ; "contrat jeune chercheur'' INSERM[00000000] ; IBiSA, Aix-Marseille Universite[00000000] ; [ANR-10-INBS-0009-10]
WOS关键词	RAT TESTIS ; ACETYLATION ; HETEROCHROMATIN ; TRANSCRIPTION ; BROMODOMAIN ; MARKS ; SPERMIOGENESIS ; IDENTIFICATION ; CROTONYLATION ; NUCLEOSOMES
WOS研究方向	Cell Biology ; Developmental Biology ; Genetics & Heredity
语种	英语
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS记录号	WOS:000322716000006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277521]
专题	化学蛋白质组学研究中心
通讯作者	Khochbin, Saadi
作者单位	1.CHU Angers, F-49000 Angers, France; 2.Univ Grenoble 1, Inst Albert Bonniot, INSERM, U823, F-38700 Grenoble, France; 3.Commissariat Energie Atom & Energies Alternat CEA, Lab Etud Metab Med, Direct Sci Vivant, Inst Biol & Technol Saclay iBiTec S,Inst Biol Tec, F-91191 Gif Sur Yvette, France; 4.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 6.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA; 7.Univ Grenoble Alpes, CNRS, CEA, Inst Biol Struct, F-38027 Grenoble, France; 8.Univ Grenoble 1, Grenoble Inst Neurosci, INSERM, U836, F-38700 Grenoble, France; 9.Univ Angers, UMR S 892, Ctr Rech Canc Nantes Angers, U892,INSERM,Plateforme SNP, F-49000 Angers, France; 10.Aix Marseille Univ, TGML TAGC, Marseille, France;
推荐引用方式 GB/T 7714	Montellier, Emilie,Boussouar, Faycal,Rousseaux, Sophie,et al. Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B[J]. GENES & DEVELOPMENT,2013,27(15):1680-1692.
APA	Montellier, Emilie.,Boussouar, Faycal.,Rousseaux, Sophie.,Zhang, Kai.,Buchou, Thierry.,...&Khochbin, Saadi.(2013).Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.GENES & DEVELOPMENT,27(15),1680-1692.
MLA	Montellier, Emilie,et al."Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B".GENES & DEVELOPMENT 27.15(2013):1680-1692.