A heteropolysaccharide, L-fuco-D-manno-1,6-alpha-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative

doi:10.1016/j.carbpol.2013.09.085

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	A heteropolysaccharide, L-fuco-D-manno-1,6-alpha-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative
	Wang, Ying 3; Shen, Xiaokun 1,2; Liao, Wenfeng 3; Fang, Jianping 3; Chen, Xia 3; Dong, Qun 3; Ding, Kan3
刊名	CARBOHYDRATE POLYMERS
	2014-01-30
卷号	101 页码:631-641
关键词	Grifola frondosa Galactan Antiangiogenesis Human microvascular endothelial cell line-1 (HMEC-1)
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2013.09.085
文献子类	Article
英文摘要	The tumor growth and metastasis are angiogenesis dependent, thus blockade of angiogenesis is a promising approach for treatment of cancer. Herein we reported the structural and biological features of a novel water-soluble polysaccharide named GFPW from the fruit body of Grifola frondosa. Chemical and spectral analysis revealed that GFPW with an average molecular weight of 15.7 kDa, possessed a backbone consisting of alpha-1,6-linked galactopyranosyl residues, with branches attached to O-2 of alpha-1,3-linked fucose residues and alpha-terminal mannose. By the chlorosulfonic acid-pyridine method, we prepared a sulfated derivative of GFPW, Sul-GFPW, with a substitution degree of 0.33. According to the C-13 NMR spectrum, the substitution position was deduced at C-2 and C-3. The angiogenesis assays in vitro showed that Sul-GFPW significantly inhibited endothelial cell proliferation in a dose- and time-dependent manner, and reduced endothelial cell migration and tube formation as well. (C) 2013 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[81171914] ; National Natural Science Foundation of China[31230022] ; National Natural Science Foundation of China[81202103] ; National Science Fund for Distinguished Young Scholars in China[81125025] ; New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; Industry-University-Research Institute Alliance Fund in Guangdong Province, China[2010A090200041]
WOS关键词	MANNO-D-GLUCAN ; STRUCTURAL-CHARACTERIZATION ; MICROELLOBOSPORIA-GRISEA ; ANTITUMOR-ACTIVITY ; PLATYCODON-GRANDIFLORUM ; PLEUROTUS-OSTREATUS ; GANODERMA-LUCIDUM ; FRUITING BODIES ; IN-VITRO ; POLYSACCHARIDE
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000330494800079
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277216]
专题	药理学第三研究室药物化学研究室
通讯作者	Ding, Kan
作者单位	1.Zhejiang Univ, Dept Med, Hangzhou 310029, Zhejiang, Peoples R China 2.Wenzhou Med Coll, Dept Oncol, Linhai 317000, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Ying,Shen, Xiaokun,Liao, Wenfeng,et al. A heteropolysaccharide, L-fuco-D-manno-1,6-alpha-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative[J]. CARBOHYDRATE POLYMERS,2014,101:631-641.
APA	Wang, Ying.,Shen, Xiaokun.,Liao, Wenfeng.,Fang, Jianping.,Chen, Xia.,...&Ding, Kan.(2014).A heteropolysaccharide, L-fuco-D-manno-1,6-alpha-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative.CARBOHYDRATE POLYMERS,101,631-641.
MLA	Wang, Ying,et al."A heteropolysaccharide, L-fuco-D-manno-1,6-alpha-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative".CARBOHYDRATE POLYMERS 101(2014):631-641.