Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways

doi:10.1038/aps.2014.70

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	Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways
	Wu, Ruo-ming 2; Sun, Yan-yan 2; Zhou, Ting-ting 1; Zhu, Zhi-yuan 1; Zhuang, Jing-jing 2; Tang, Xuan 1; Chen, Jing1 ; Hu, Li-hong 1; Shen, Xu1,2
刊名	ACTA PHARMACOLOGICA SINICA
	2014-10
卷号	35 期号:10 页码:1274-1284
关键词	arctigenin skeletal muscle weight-loaded forced swimming test fatigue physical endurance ROS antioxidant AMPK PPAR alpha Nrf2
ISSN号	1671-4083
DOI	10.1038/aps.2014.70
文献子类	Article
英文摘要	Aim: Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignan found in traditional Chinese herbs, has been determined to exhibit a variety of pharmacological activities, including anti-tumor, anti-inflammation, neuroprotection, and endurance enhancement. In the present study, we investigated the antioxidation and anti-fatigue effects of arctigenin in rats. Methods: Rat L6 skeletal muscle cell line was exposed to H2O2 (700 mu mol/L), and ROS level was assayed using DCFH-DA as a probe. Male SD rats were injected with arctigenin (15 mg.kg(-1).d(-1),ip) for 6 weeks, and then the weight-loaded forced swimming test (WFST) was performed to evaluate their endurance. The levels of antioxidant-related genes in L6 cells and the skeletal muscles of rats were analyzed using real-time RT-PCR and Western blotting. Results: Incubation of L6 cells with arctigenin (1, 5, and 20 mu mol/L) dose-dependently decreased the H2O2-induced ROS production. WFST results demonstrated that chronic administration of arctigenin significantly enhanced the endurance of rats. Furthermore, molecular biology studies on L6 cells and skeletal muscles of the rats showed that arctigenin effectively increased the expression of the antioxidant-related genes, including superoxide dismutase (SOD), glutathione reductase (Gsr), glutathione peroxidase (GPX1), thioredoxin (Txn) and uncoupling protein 2 (UCP2), through regulation of two potential antioxidant pathways: AMPK/PGC-1 alpha/PPAR alpha in mitochondria and AMPK/p53/Nrf2 in the cell nucleus. Conclusion: Arctigenin efficiently enhances rat swimming endurance by elevation of the antioxidant capacity of the skeletal muscles, which has thereby highlighted the potential of this natural product as an antioxidant in the treatment of fatigue and related diseases.
资助项目	National Natural Science Foundation of China[81220108025] ; National Natural Science Foundation of China[81373461] ; Science and Technology Commission of Shanghai Municipality[12431900300]
WOS关键词	ACTIVATED PROTEIN-KINASE ; OXIDATIVE STRESS ; UNCOUPLING PROTEIN-2 ; SKELETAL-MUSCLE ; UP-REGULATION ; INDUCED NEUROTOXICITY ; RAW264.7 CELLS ; EXERCISE ; FATIGUE ; INJURY
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:5244401
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000342734800005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276883]
专题	药物安全性评价中心药理学第三研究室
通讯作者	Chen, Jing
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;
推荐引用方式 GB/T 7714	Wu, Ruo-ming,Sun, Yan-yan,Zhou, Ting-ting,et al. Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways[J]. ACTA PHARMACOLOGICA SINICA,2014,35(10):1274-1284.
APA	Wu, Ruo-ming.,Sun, Yan-yan.,Zhou, Ting-ting.,Zhu, Zhi-yuan.,Zhuang, Jing-jing.,...&Shen, Xu.(2014).Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways.ACTA PHARMACOLOGICA SINICA,35(10),1274-1284.
MLA	Wu, Ruo-ming,et al."Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways".ACTA PHARMACOLOGICA SINICA 35.10(2014):1274-1284.