Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity

doi:10.1016/j.carbpol.2015.05.004

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	Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity
	Zheng, Changping 1,2; Dong, Qun 2; Chen, Huanjun 2; Cong, Qifei 2; Ding, Kan2
刊名	CARBOHYDRATE POLYMERS
	2015-10-05
卷号	130 页码:113-121
关键词	Chrysanthemum morifolium Polysaccharide Structural elucidation Antioxidant
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2015.05.004
文献子类	Article
英文摘要	The flowers of Chrysanthemum morifolium were extracted by boiling water, and a water-soluble polysaccharide (CMJA0S2) with a molecular weight of 6.5 kDa was isolated by anion-exchange chromatography on a DEAE-cellulose column and gel permeation chromatography on a Sephacryl S-300 HR column. Monosaccharide composition analysis indicated that CMJA0S2 was composed of galactose, glucose, mannose and arabinose in molar ratio of 4.1:3.3: 1.0: 2.3. According to linkage analysis, partial acid hydrolysis and NMR spectra, the backbone was shown to contain 1, 4-linked P-Galp, 1, 4-linked beta-Glcp and 1, 4-linked beta-Manp, with branches substituted at C-6 of 1, 4-linked beta-Manp by I, 6-linked beta-Galp and at 0-6 of partial 1, 4-linked P-Galp substituted by T-linked alpha-Glcp. About 40% of 1, 6-linked beta-Galp with T-linked alpha-Glcp was substituted at O-3 by alpha-Araf-(1 -> [5)-alpha-Araf-(1](3). The anti-oxidative analysis showed that CMJA0S2 could scavenge the DPPH radicals and relieve the damage of PC12 cells caused by H2O2, thus it could be regarded as a potential natural antioxidant. (C) 2015 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China (NSFC)[31230022] ; New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; National Science Fund for Distinguished Young Scholars in China[81125025]
WOS关键词	WATER-SOLUBLE POLYSACCHARIDE ; CASSIA-OBTUSIFOLIA SEEDS ; FRUIT BODIES ; ARABINOGALACTAN ; FEATURES ; ROOTS ; PURIFICATION ; THUNB
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000357244900014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276370]
专题	药理学第三研究室
通讯作者	Dong, Qun
作者单位	1.Nan Chang Univ, Coll Pharm, Nanchang 330000, Jiangxi, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycobiol & Glycochem Lab, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Changping,Dong, Qun,Chen, Huanjun,et al. Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity[J]. CARBOHYDRATE POLYMERS,2015,130:113-121.
APA	Zheng, Changping,Dong, Qun,Chen, Huanjun,Cong, Qifei,&Ding, Kan.(2015).Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity.CARBOHYDRATE POLYMERS,130,113-121.
MLA	Zheng, Changping,et al."Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity".CARBOHYDRATE POLYMERS 130(2015):113-121.