Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity

doi:10.1016/j.ijbiomac.2016.03.025

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity
	Lin, Liyan 1,2; Wang, Peipei1 ; Du, Zhenyun 1; Wang, Wucheng 1; Cong, Qifei 1; Zheng, Changping 1; Jin, Can 1,2; Ding, Kan1,2 ; Shao, Chenghao 3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2016-07
卷号	88 页码:130-137
关键词	Lonicera japonica Polysaccharide Antipancreatic cancer cells growth
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2016.03.025
文献子类	Article
英文摘要	To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L.japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54 kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2 M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked beta-D-Galp, T- and 1,5-linked alpha-L-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1 mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	State Key Program of National Natural Science Foundation of China[31230022] ; New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; Natural Science Foundation of China[31400690] ; National Science Fund for Distinguished Young Scholars in China[81125025]
WOS关键词	CHRYSANTHEMUM-MORIFOLIUM FLOWERS ; RHAMNOGALACTURONAN I ; CELL-PROLIFERATION ; POLYSACCHARIDE ; ARABINOGALACTAN
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000376800600016
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275980]
专题	药理学第三研究室
通讯作者	Ding, Kan; Shao, Chenghao
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China; 2.Zunyi Med Univ, Sch Pharm, 201 Dalian Rd, Zunyi 563003, Peoples R China; 3.Shanghai Changzheng Hosp, Dept Gen Surg, 415 Fengyang Rd, Shanghai 200003, Peoples R China
推荐引用方式 GB/T 7714	Lin, Liyan,Wang, Peipei,Du, Zhenyun,et al. Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,88:130-137.
APA	Lin, Liyan.,Wang, Peipei.,Du, Zhenyun.,Wang, Wucheng.,Cong, Qifei.,...&Shao, Chenghao.(2016).Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,88,130-137.
MLA	Lin, Liyan,et al."Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 88(2016):130-137.