Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification

doi:10.1016/j.carres.2017.07.011

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	Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification
	Cai, Deqin 3,4; Xun, Chaochao 2,4; Tang, Feng 3,4; Tian, Xiaobo 4; Yang, Liyun 4; Ding, Kan4 ; Li, Wenzhe 1; Le, Zhiping 2; Huang, Wei 3,4
刊名	CARBOHYDRATE RESEARCH
	2017-09-08
卷号	449 页码:143-152
关键词	Core-fucose Glycoconjugate Glyco-dendrimer Glyco-agarose Fucose-specific lectin
ISSN号	0008-6215
DOI	10.1016/j.carres.2017.07.011
文献子类	Review
英文摘要	Glyco-PAMAM dendrimers and glyco-agarose beads bearing a core-fucosylated N-glycan trisaccharide GlcNAc beta 1,4(Fuc alpha 1,6) GlcNAc or a non-fucose disaccharide GlcNAc beta 1,4GlcNAc were successfully synthesized and characterized by monosaccharide analysis with HPAEC-PAD technique. These glycoconjugates as fucose lectin probes were applied in fucose-specific lectin detection and purification. The model fucose lectin AAL indicated binding activity with the FITC-labeled PAMAM carrying core-fucose trisaccharide. An affinity chromatography column stuffed with the agarose beads carrying core-fucosylated trisaccharide exhibited a good specificity in purification of AAL than non-fucose disaccharide agarose beads. These novel glycoconjugates bearing the precise and simplified core-fucose N-glycan structure provided a potential application for core-fucose-specific lectin discovery. (C) 2017 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China (NNSFC)[21372238] ; National Natural Science Foundation of China (NNSFC)[21572244] ; National Natural Science Foundation of China (NNSFC)[31570797] ; SIMM institute fund[CASIMM0120153004] ; "Personalized Medicines: Molecular Signature-based Drug Discovery and Development", Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020311]
WOS关键词	ALPHA-FETOPROTEIN ; HEPATOCELLULAR-CARCINOMA ; PSEUDOMONAS-AERUGINOSA ; HIGH-AFFINITY ; GLYCOSYLATION ; BINDING ; LIGANDS ; BLOOD ; ACIDS ; IGG
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000410830000017
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272485]
专题	生物技术药物研发中心（筹）药物安全性评价中心
通讯作者	Li, Wenzhe; Huang, Wei
作者单位	1.Dalian Med Univ, Coll Basic Med Sci, 9 Western Sect,Lvshun South Rd, Dalian 116044, Liaoning, Peoples R China 2.Nanchang Univ, Dept Chem, 999 Xuefu Ave, Nanchang 330031, Jiangxi, Peoples R China; 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Cai, Deqin,Xun, Chaochao,Tang, Feng,et al. Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification[J]. CARBOHYDRATE RESEARCH,2017,449:143-152.
APA	Cai, Deqin.,Xun, Chaochao.,Tang, Feng.,Tian, Xiaobo.,Yang, Liyun.,...&Huang, Wei.(2017).Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification.CARBOHYDRATE RESEARCH,449,143-152.
MLA	Cai, Deqin,et al."Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification".CARBOHYDRATE RESEARCH 449(2017):143-152.