Separation and analysis of sialyglycopeptides extracted from egg yolks

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	Separation and analysis of sialyglycopeptides extracted from egg yolks
	Li Mingjie 1; Tang Feng 3; Guo Weilai 3; Xu Liying 2; Huang Wei 3
刊名	Journal of Shenyang Pharmaceutical University
	2018
卷号	35 期号:9 页码:769-775
关键词	Hilic column-high performance liquid chromatography sialylglycopeptide monosialylglycopeptide single factor optimization method
ISSN号	1006-2858
其他题名	蛋黄及其提取物中唾液酸糖肽的分离与测定
文献子类	Article
英文摘要	Objective To establish the HPLC analysis method of sialylglycopeptide (SGP) , to optimize the extraction process. Methods Harnessing a Hilic hydrophilic column,a C18 reversed-phase column,and a high-performance anion-exchange column; Optimizing HPLC elution conditions to analyze sialylglycopeptide crude samples,and then compare and optimize the separation of dual-sialylglycopeptide (d-SGP) and monosialylglycopeptide (m-SGP) using three different HPLC columns; The separation efficiency was evaluated based on peak shape, retention time interval,peak symmetry,and separation resolution. Then single factor optimization method was adopted to evaluate the extraction rate of d-SGP. The extraction times,amount of ethanol,extration duration were selected as the optimization factors to optimize the extraction process. Results Under optimal HPLC conditions, the separation of d-SGP and m-SGP indicated better resolution with the Hilic column and the anion-exchange column than with the C18 column. The optimal extraction process is using 60% ethyl alcohol as the extracting solution,extracting 60 min each time for 3 times at a ratio of egg yolk powder: extracting solution (1: 5) . Conclusion In the study of the separation of sialylglycopeptides,the Hilic chromatographic column is the best for the separation of SGP samples. The optimized extraction process is feasible.
资助项目	国家自然科学基金资助项目[00000000]
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:6341753
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269154]
专题	生物技术药物研发中心（筹）药物安全性评价中心
作者单位	1.Shenyang Pharmaceutical University; 2.Shenyang Pharmaceutical University, Ministry of Education Key Laboratory of Structure-Based Drug Design and Discovery, Shenyang, Liaoning 110016, China. 3.Chinese Academy of Sciences Shanghai Institute of Materia Medical, Shanghai 201203, China.; 4.; 5.Shenyang; 6.Ministry of Education Key Laboratory of Structure-Based Drug Design and Discovery;
推荐引用方式 GB/T 7714	Li Mingjie,Tang Feng,Guo Weilai,et al. Separation and analysis of sialyglycopeptides extracted from egg yolks[J]. Journal of Shenyang Pharmaceutical University,2018,35(9):769-775.
APA	Li Mingjie,Tang Feng,Guo Weilai,Xu Liying,&Huang Wei.(2018).Separation and analysis of sialyglycopeptides extracted from egg yolks.Journal of Shenyang Pharmaceutical University,35(9),769-775.
MLA	Li Mingjie,et al."Separation and analysis of sialyglycopeptides extracted from egg yolks".Journal of Shenyang Pharmaceutical University 35.9(2018):769-775.