Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum

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	Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum
	WANG Saizhen 1; DING Kan3 ; LIN Shuqian 1; LIN Zhibin 1
刊名	Acta Pharmaceutica Sinica
	2007
卷号	42 期号:10 页码:1058-1061
关键词	Ganoderma lucidum polysaccharide peptide structure
ISSN号	0513-4870
其他题名	赤芝多糖肽GL-PP-3A的分离纯化和结构研究
文献子类	Article
英文摘要	GL-PP-3A, an active polysaccharide peptide, was isolated and purified from Ganoderma lucidum, and then its structure was analyzed. Crude polysaccharide peptides were extracted from Ganoderma lucidum with hot water, precipitated with ethanol and then dialyzed from Ganoderma lucidum. Subsequently GL-PP-3A was isolated and purified from the crude polysaccharide peptides by fractional precipitation and chromatography of Bio-Gel P-10 column. The repetitive unit of GL-PP-3A was analyzed by high performance gel permeation chromatography （HPGPC）, monosaccharide composition and methylation analysis, ~1H NMR and ~(13)C NMR. GL-PP-3A is a heteropolysaccharide which is composed mainly of glucose （Glc）, and also contains saccharide residues such as rhamnose （Rha）, xylose （Xyl） , mannose （Man） and galactose （Gal） and 17 kinds of amino acids. Its weight-average molecular weight （Mw） and number-average molecular weight （Mn） were 1.7 * 10~4 and 1.1 * 10~4, respectively, with the ratio of Mw/Mm （ molecular weight distribution） being of 1.49. Its backbone chain is composed of 1,6-linked beta-D-Glcp and 1,3-liked beta-D-Glcp at a ratio of 2 ： 1. Some of 1,6-linked glucose residuals of the backbone chain are substituted at 2-0 or 3-0, and there are 1 to 3 1,6-linked beta-D-Galp or 1,3-linked alpha-D-Manp in the branched chains, the nonreducing ends of which consist mainly of beta-D-Glcp and a few Rha.
WOS研究方向	General & Internal Medicine (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:2927310
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268164]
专题	药理学第三研究室
作者单位	1.Fuzhou Green Valley Biopharmaceutical Research Institute, Fuzhou, Fujian 350002, China.; 2.Shanghai 201203, China. 3.Shanghai Institute of Materia Medica;
推荐引用方式 GB/T 7714	WANG Saizhen,DING Kan,LIN Shuqian,et al. Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum[J]. Acta Pharmaceutica Sinica,2007,42(10):1058-1061.
APA	WANG Saizhen,DING Kan,LIN Shuqian,&LIN Zhibin.(2007).Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum.Acta Pharmaceutica Sinica,42(10),1058-1061.
MLA	WANG Saizhen,et al."Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum".Acta Pharmaceutica Sinica 42.10(2007):1058-1061.