Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum | |
WANG Saizhen1; DING Kan3![]() | |
刊名 | Acta Pharmaceutica Sinica
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2007 | |
卷号 | 42期号:10页码:1058-1061 |
关键词 | Ganoderma lucidum polysaccharide peptide structure |
ISSN号 | 0513-4870 |
其他题名 | 赤芝多糖肽GL-PP-3A的分离纯化和结构研究 |
文献子类 | Article |
英文摘要 | GL-PP-3A, an active polysaccharide peptide, was isolated and purified from Ganoderma lucidum, and then its structure was analyzed. Crude polysaccharide peptides were extracted from Ganoderma lucidum with hot water, precipitated with ethanol and then dialyzed from Ganoderma lucidum. Subsequently GL-PP-3A was isolated and purified from the crude polysaccharide peptides by fractional precipitation and chromatography of Bio-Gel P-10 column. The repetitive unit of GL-PP-3A was analyzed by high performance gel permeation chromatography (HPGPC), monosaccharide composition and methylation analysis, ~1H NMR and ~(13)C NMR. GL-PP-3A is a heteropolysaccharide which is composed mainly of glucose (Glc), and also contains saccharide residues such as rhamnose (Rha), xylose (Xyl) , mannose (Man) and galactose (Gal) and 17 kinds of amino acids. Its weight-average molecular weight (Mw) and number-average molecular weight (Mn) were 1.7 * 10~4 and 1.1 * 10~4, respectively, with the ratio of Mw/Mm ( molecular weight distribution) being of 1.49. Its backbone chain is composed of 1,6-linked beta-D-Glcp and 1,3-liked beta-D-Glcp at a ratio of 2 : 1. Some of 1,6-linked glucose residuals of the backbone chain are substituted at 2-0 or 3-0, and there are 1 to 3 1,6-linked beta-D-Galp or 1,3-linked alpha-D-Manp in the branched chains, the nonreducing ends of which consist mainly of beta-D-Glcp and a few Rha. |
WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
语种 | 中文 |
CSCD记录号 | CSCD:2927310 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/268164] ![]() |
专题 | 药理学第三研究室 |
作者单位 | 1.Fuzhou Green Valley Biopharmaceutical Research Institute, Fuzhou, Fujian 350002, China.; 2.Shanghai 201203, China. 3.Shanghai Institute of Materia Medica; |
推荐引用方式 GB/T 7714 | WANG Saizhen,DING Kan,LIN Shuqian,et al. Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum[J]. Acta Pharmaceutica Sinica,2007,42(10):1058-1061. |
APA | WANG Saizhen,DING Kan,LIN Shuqian,&LIN Zhibin.(2007).Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum.Acta Pharmaceutica Sinica,42(10),1058-1061. |
MLA | WANG Saizhen,et al."Isolation,purification and structural analysis of GL-PP-3A,an active polysaccharide peptide from Ganoderma lucidum".Acta Pharmaceutica Sinica 42.10(2007):1058-1061. |
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