Purification and Analysis of Male Germ Cells from Adult Mouse Testis

doi:10.1007/978-1-4939-6527-4_12

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	Purification and Analysis of Male Germ Cells from Adult Mouse Testis
	Buchou, Thierry 4; Tan, Minjia1 ; Barral, Sophie 3; Vitte, Anne-Laure 3; Rousseaux, Sophie 3; Arechaga, Juan 2; Khochbin, Saadi 3
刊名	Methods in molecular biology (Clifton, N.J.)
	2017
卷号	1510 页码:159-168
ISSN号	1940-6029
DOI	10.1007/978-1-4939-6527-4_12
文献子类	Article
英文摘要	Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purification of meiotic and post-meiotic male germ cells from adult mouse testes. We also describe how the fractionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266814]
专题	化学蛋白质组学研究中心
通讯作者	Buchou, Thierry
作者单位	1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; 2.Laboratory of Stem Cells, Development and Cancer, Department of Cell Biology and Histology, University of the Basque Country (UPV/EHU), Vizcaya, Spain 3.CNRS UMR 5209, INSERM U1209, CR UGA, Institute for Advanced Biosciences, La Tronche, 38700, France; 4.CNRS UMR 5209, INSERM U1209, CR UGA, Institute for Advanced Biosciences, La Tronche, 38700, France. thierry;
推荐引用方式 GB/T 7714	Buchou, Thierry,Tan, Minjia,Barral, Sophie,et al. Purification and Analysis of Male Germ Cells from Adult Mouse Testis[J]. Methods in molecular biology (Clifton, N.J.),2017,1510:159-168.
APA	Buchou, Thierry.,Tan, Minjia.,Barral, Sophie.,Vitte, Anne-Laure.,Rousseaux, Sophie.,...&Khochbin, Saadi.(2017).Purification and Analysis of Male Germ Cells from Adult Mouse Testis.Methods in molecular biology (Clifton, N.J.),1510,159-168.
MLA	Buchou, Thierry,et al."Purification and Analysis of Male Germ Cells from Adult Mouse Testis".Methods in molecular biology (Clifton, N.J.) 1510(2017):159-168.