Quantitative Proteomic Study Reveals Up-Regulation of cAMP Signaling Pathway-Related Proteins in Mild Traumatic Brain Injury

doi:10.1021/acs.jproteome.7b00618

	Quantitative Proteomic Study Reveals Up-Regulation of cAMP Signaling Pathway-Related Proteins in Mild Traumatic Brain Injury
	Song, Hai 1,4; Fang, Shanhua 2; Gao, Jing 3,6; Wang, Jiaxong 1,4; Can, Zhenzhen 5; Guo, Zeyun 5; Huang, Qiongping 3,6; Qu, Yongqang 4; Zhou, Hu2,3,6 ; Yu, Jianyun 4
刊名	JOURNAL OF PROTEOME RESEARCH
	2018-02
卷号	17 期号:2 页码:858-869
关键词	mTBI iTRAQ proteomic analysis cAMP signal process Pde10a Gnal
ISSN号	1535-3893
DOI	10.1021/acs.jproteome.7b00618
文献子类	Article
英文摘要	Traumatic brain injury (TBI), as a neurological injury, becomes a leading cause of disability and mortality due to lacking effective therapy. About 75% of TBI is mild traumatic brain injury (mTBI). However, the complex molecular mechanisms underlying mTBI pathophysiology remains to be elucidated. In this study, iTRAQ-based quantitative proteomic approach was employed to measure temporal-global proteome changes of rat brain tissues from different time points (1 day, 7 day and 6 months) post single mTBI (smTBI) and repetitive mTBI (rmTBI). A total of 5169 proteins were identified, of which, 237 proteins were significantly changed between control rats and mTBI model rats. Fuzzy c-means (FCM) clustering analysis classified these 237 proteins into six clusters according to their temporal pattern of protein abundance. Functional bioinformatics analysis and protein-protein interaction (PPI) network mapping of these FCM clusters showed that phosphodiesterase 10A (Pde10a) and guanine nucleotide-binding protein G (olf) subunit alpha (Gnal) were the node proteins in the cAMP signaling pathway. Other biological processes, such as cell adhesion, autophagy, myelination, microtubule depolymerization and brain development, were also over-represented in FCM clusters. Further Western Blot experiments confirmed that Pde10a and Gnal were acutely up-regulated in severity-dependent manner by mTBI, but these two proteins could not be down-regulated to basal level at the time point of 6 months post repetitive mTBI. Our study demonstrated that different severity of mTBI cause significant temporal profiling change at the proteomic level and pointed out the cAMP signaling pathway-related proteins, Pde10a and Gnal, may play important roles in the pathogenesis and recovery of mTBI.
资助项目	National Nature Science Foundation of China[81360467] ; National Nature Science Foundation of China[21375138] ; Key Project of Application and Basis Research of Yunnan Province, China[2013FB104] ; Chinese Academy of Sciences[00000000] ; Chinese Academy of Sciences[XDA12030203]
WOS关键词	TANDEM MASS-SPECTROMETRY ; ITRAQ ; PDE10A ; PHOSPHODIESTERASE ; EXPRESSION ; STRIATUM ; CELLS ; PHOSPHORYLATION ; CHROMATOGRAPHY ; DEGENERATION
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000424730700010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279929]
专题	分析化学研究室
通讯作者	Zhou, Hu; Yu, Jianyun
作者单位	1.Kunming Med Univ, Affiliated Hosp 1, Dept Neurosurg, Kunming 650032, Yunnan, Peoples R China 2.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 4.Kunming Med Univ, Dept Forens Med, Kunming 650032, Yunnan, Peoples R China; 5.Kunming Med Univ, Dept Anat & Histol, Kunming 650032, Yunnan, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Song, Hai,Fang, Shanhua,Gao, Jing,et al. Quantitative Proteomic Study Reveals Up-Regulation of cAMP Signaling Pathway-Related Proteins in Mild Traumatic Brain Injury[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(2):858-869.
APA	Song, Hai.,Fang, Shanhua.,Gao, Jing.,Wang, Jiaxong.,Can, Zhenzhen.,...&Yu, Jianyun.(2018).Quantitative Proteomic Study Reveals Up-Regulation of cAMP Signaling Pathway-Related Proteins in Mild Traumatic Brain Injury.JOURNAL OF PROTEOME RESEARCH,17(2),858-869.
MLA	Song, Hai,et al."Quantitative Proteomic Study Reveals Up-Regulation of cAMP Signaling Pathway-Related Proteins in Mild Traumatic Brain Injury".JOURNAL OF PROTEOME RESEARCH 17.2(2018):858-869.