A binding-block ion selective mechanism revealed by a Na/K selective channel

doi:10.1007/s13238-017-0465-8

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第二研究室

	A binding-block ion selective mechanism revealed by a Na/K selective channel
	Yu, Jie 6; Zhang, Bing 1,2,4; Zhang, Yixiao 6; Xu, Cong-qiao 3,5; Zhuo, Wei 6; Ge, Jingpeng 6; Li, Jun3,5 ; Gao, Ning 6; Li, Yang4 ; Yang, Maojun 6
刊名	PROTEIN & CELL
	2018-07
卷号	9 期号:7 页码:629-639
关键词	cryo-EM MscS Na+/K+ selective channel
ISSN号	1674-800X
DOI	10.1007/s13238-017-0465-8
文献子类	Article
英文摘要	Mechanosensitive (MS) channels are extensively studied membrane protein for maintaining intracellular homeostasis through translocating solutes and ions across the membrane, but its mechanisms of channel gating and ion selectivity are largely unknown. Here, we identified the YnaI channel as the Na+/K+ cation-selective MS channel and solved its structure at 3.8 by cryo-EM single-particle method. YnaI exhibits low conductance among the family of MS channels in E. coli, and shares a similar overall heptamer structure fold with previously studied MscS channels. By combining structural based mutagenesis, quantum mechanical and electrophysiological characterizations, we revealed that ion selective filter formed by seven hydrophobic methionine (YnaI(Met158)) in the transmembrane pore determined ion selectivity, and both ion selectivity and gating of YnaI channel were affected by accompanying anions in solution. Further quantum simulation and functional validation support that the distinct binding energies with various anions to YnaI(Met158) facilitate Na+/K+ pass through, which was defined as binding-block mechanism. Our structural and functional studies provided a new perspective for understanding the mechanism of how MS channels select ions driven by mechanical force.
资助项目	Ministry of Science and Technology[2016YFA0501100] ; Ministry of Science and Technology[2017YFA0504600] ; Ministry of Science and Technology[2016YFA0500700] ; Ministry of Science and Technology[2013CB910400] ; National Fund for Distinguished Young Scholar[31625008] ; National Natural Science Foundation of China[21532004] ; National Natural Science Foundation of China[31570733] ; National Natural Science Foundation of China[31422016] ; National Natural Science Foundation of China[31371066] ; National Natural Science Foundation of China[31671049] ; National Natural Science Foundation of China[91426302]
WOS关键词	GATED SODIUM-CHANNEL ; CRYOELECTRON MICROSCOPY STRUCTURE ; CRYSTAL-STRUCTURE ; MECHANOSENSITIVE CHANNEL ; K+ CHANNEL ; CRYO-EM ; RESOLUTION ; COMPLEX ; MSCS ; ARCHITECTURE
WOS研究方向	Cell Biology
语种	英语
CSCD记录号	CSCD:6321830
出版者	HIGHER EDUCATION PRESS
WOS记录号	WOS:000436455300004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279687]
专题	药理学第二研究室
通讯作者	Gao, Ning; Li, Yang; Yang, Maojun
作者单位	1.Tongji Univ, Sch Med, Shanghai Matern & Infant Hosp 1, Anesthesiol, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Tsinghua Univ, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China; 5.Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China; 6.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Minist Educ, Key Lab Prot Sci,Sch Life Sci,Tsinghua Peking Joi, Beijing 100084, Peoples R China;
推荐引用方式 GB/T 7714	Yu, Jie,Zhang, Bing,Zhang, Yixiao,et al. A binding-block ion selective mechanism revealed by a Na/K selective channel[J]. PROTEIN & CELL,2018,9(7):629-639.
APA	Yu, Jie.,Zhang, Bing.,Zhang, Yixiao.,Xu, Cong-qiao.,Zhuo, Wei.,...&Yang, Maojun.(2018).A binding-block ion selective mechanism revealed by a Na/K selective channel.PROTEIN & CELL,9(7),629-639.
MLA	Yu, Jie,et al."A binding-block ion selective mechanism revealed by a Na/K selective channel".PROTEIN & CELL 9.7(2018):629-639.