Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography

doi:10.1002/pca.2777

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 天然药物化学研究室

	Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography
	Ma, Yaqian 2,4; Liu, Menghan 1; Tan, Ting 3; Yan, Aiping 3; Guo, Lan 3; Jiang, Kun 4; Tan, Changheng4 ; Wan, Yiqun 2,3
刊名	PHYTOCHEMICAL ANALYSIS
	2018-11
卷号	29 期号:6 页码:639-648
关键词	Box-Behnken design Camellia oleifera flowers deep eutectic solvents flavonoids response surface methodology
ISSN号	0958-0344
DOI	10.1002/pca.2777
文献子类	Article
英文摘要	Introduction: Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. Objective: To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection. Methodology: Preparing an environmentally-friendly and effective solvent - deep eutectic solvents (DESs) - for compound extraction. Then investigating the influential factors of ultrasound-assisted extraction with DESs by the Box-Behnken design combined with response surface methodology. Results: DES-5 synthesised with choline chloride and lactic acid (1:2) acquired excellent extractability for four flavonoids (quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside and their aglycones) with different polarity. The proposed method, which could simultaneously determine four flavonoids with HPLC-UV detection for the first time, displays satisfactory recovery yields and high precision with inter-day relative standard deviation lower than 5.80%. Conclusion: DESs could be promising solvents for efficiently and selectively extracting bioactive compounds from plant materials, and the analytical method for flavonoids of C. oleifera flowers could provide reference value for its application and be used in other plant resources.
资助项目	Natural Science Foundation of P. R. China[31660483] ; Research Programme of the State Key Laboratory of Food Science and Technology in Nanchang University[SKLF-KF-201617]
WOS关键词	PHENOLIC-COMPOUNDS ; EFFICIENT EXTRACTION ; BIOACTIVE FLAVONOIDS ; CHOLINE CHLORIDE ; GREEN EXTRACTION ; OPTIMIZATION ; QUERCETIN ; MICROEXTRACTION ; ULTRASOUND ; GLYCOSIDES
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
语种	英语
出版者	WILEY
WOS记录号	WOS:000446458200010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279524]
专题	天然药物化学研究室
通讯作者	Tan, Changheng; Wan, Yiqun
作者单位	1.Nanchang Univ, Clin Med Coll 2, Nanchang, Jiangxi, Peoples R China; 2.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China; 3.Nanchang Univ, Ctr Anal & Testing, 235 East Nanjing Rd, Nanchang 330029, Jiangxi, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Nat Med Chem, Shanghai, Peoples R China;
推荐引用方式 GB/T 7714	Ma, Yaqian,Liu, Menghan,Tan, Ting,et al. Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography[J]. PHYTOCHEMICAL ANALYSIS,2018,29(6):639-648.
APA	Ma, Yaqian.,Liu, Menghan.,Tan, Ting.,Yan, Aiping.,Guo, Lan.,...&Wan, Yiqun.(2018).Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography.PHYTOCHEMICAL ANALYSIS,29(6),639-648.
MLA	Ma, Yaqian,et al."Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography".PHYTOCHEMICAL ANALYSIS 29.6(2018):639-648.