The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence

doi:10.1016/j.bbrc.2018.10.065

	The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence
	Meng, Qian 1,2,3; Gao, Jing 1,2; Zhu, Hongwen 1,2; He, Han 1,2; Lu, Zhi 4; Hong, Minhua 4; Zhou, Hu1,2,3
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2018-11-10
卷号	505 期号:4 页码:1112-1120
关键词	Proteomics Replicative senescence Human skin fibroblast cells SMC2 SMC4
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2018.10.065
文献子类	Article
英文摘要	Dermal fibroblast is one of the major constitutive cells of skin and plays a central role in skin senescence. The replicative senescence of fibroblasts may cause skin aging, bad wound healing, skin diseases and even cancer. In this study, a label-free quantitative proteomic approach was employed to analyzing the serial passaged human skin fibroblast (CCD-1079Sk) cells, resulting in 3371 proteins identified. Of which, 280 proteins were significantly changed in early passage (6 passages, P6), middle passage (12 passages, P12) and late passage (21 passages, P21), with a time-dependent decrease or increase tendency. Bioinformatic analysis demonstrated that the chromosome condensin complex, including structural maintenance of chromosomes protein 2 (SMC2) and structural maintenance of chromosomes protein 4 (SMC4), were down-regulated in the serially passaged fibroblast cells. The qRT-PCR and Western Blot experiments confirmed that the expression of these two proteins were significantly down-regulated in a time-dependent manner in the subculture of human skin fibroblasts (HSFb cells). In summary, we used serially passaged human skin fibroblast cells coupled with quantitative proteomic approach to profile the protein expression pattern in the temporal progress of replicative senescence in HSFb cells and revealed that the down-regulation of the chromosome condensin complex subunits, such as SMC2 and SMC4, may play an important role in the fibroblast senescence. (C) 2018 Elsevier Inc. All rights reserved.
WOS关键词	NUCLEAR-PORE COMPLEX ; INDUCED PREMATURE SENESCENCE ; HUMAN-DIPLOID FIBROBLASTS ; DNA-REPLICATION ; SPLICING FACTOR ; IN-VITRO ; EXPRESSION ; TELOMERES ; ISG15
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000450019400026
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279495]
专题	分析化学研究室
通讯作者	Lu, Zhi; Hong, Minhua; Zhou, Hu
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.Shanghai Inoherb Co Ltd, Technol Ctr, 121 Chengyin Rd, Shanghai 200083, Peoples R China;
推荐引用方式 GB/T 7714	Meng, Qian,Gao, Jing,Zhu, Hongwen,et al. The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,505(4):1112-1120.
APA	Meng, Qian.,Gao, Jing.,Zhu, Hongwen.,He, Han.,Lu, Zhi.,...&Zhou, Hu.(2018).The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,505(4),1112-1120.
MLA	Meng, Qian,et al."The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 505.4(2018):1112-1120.