Study on Metabonome in Human Serum with Hepatocellular Carcinoma or Liver Cirrhosis

	Study on Metabonome in Human Serum with Hepatocellular Carcinoma or Liver Cirrhosis
	Lu Qiang 1; Huang Yi-Hong 1; Cong Hui 1; Liu Xia2 ; Gao Hong-Chang 2; Lin Dong-Hai 2; Wang Hui-Min 1
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2009-02
卷号	37 期号:2 页码:194-198
关键词	Hepatocellular carcinoma liver cirrhosis serum metabonome nuclear magnetic resonance spectroscopy pattern recognition
ISSN号	0253-3820
文献子类	Article
英文摘要	A comparison for the metabonome in serum samples from patients of LC(liver cirrhosis), HCC(hepatocellular carcinoma) and healthy volunteers were studied with the method of NMR(nuclear magnetic resonance spectroscopy). To explore the potential of applying H-1 NMR-based metabonomics to diagnose HCC, PLS-DA (partial least square-discriminant analysis) was employed to perform the pattern recognition analysis of NMR spectra. In comparison with healthy volunteers, the result show that patients of LC and HCC were characterized by depleted levels of lipids (LDL, low-density lipoprotein and VLDL, very low-density lipoprotein), choline, acetoacetate, etc. and by elevated levels of glutamine, pyruvate, phenylalanine, tyrosine, etc.. The results of PLS-DA analysis shows that HCC patients could be discriminated from healthy volunteers and LC patients with diagnositic sensitivity of 92.1% and false positive rate of 5.7% These were better than the results of serum AFP (alpha-fetoprotein) detection. The results also show that H-1 NMR-based metabonomics couple with PLS-DA analysis is useful for clinical diagnosis of HCC, and the method has the superiorities of sensitiveness, accuracy and good reproducibility.
WOS关键词	BLOOD-PLASMA ; SPECTROSCOPY
WOS研究方向	Chemistry
语种	中文
CSCD记录号	CSCD:3576811
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000265169000006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279326]
专题	分析化学研究室药物安全性评价中心
通讯作者	Wang Hui-Min
作者单位	1.Nantong Univ, Hosp Nantong Univ, Dept Lab Med, Key Sci Jiangsu Lab Diagnost, Nantong 226001, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Lu Qiang,Huang Yi-Hong,Cong Hui,et al. Study on Metabonome in Human Serum with Hepatocellular Carcinoma or Liver Cirrhosis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(2):194-198.
APA	Lu Qiang.,Huang Yi-Hong.,Cong Hui.,Liu Xia.,Gao Hong-Chang.,...&Wang Hui-Min.(2009).Study on Metabonome in Human Serum with Hepatocellular Carcinoma or Liver Cirrhosis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(2),194-198.
MLA	Lu Qiang,et al."Study on Metabonome in Human Serum with Hepatocellular Carcinoma or Liver Cirrhosis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.2(2009):194-198.