Proteomic reactors and their applications in biology

doi:10.1111/j.1742-4658.2011.08292.x

	Proteomic reactors and their applications in biology
	Zhou, Hu1,2,3 ; Ning, Zhibin 1,3; Wang, Fangjun 1,3,4; Seebun, Deeptee 1,3; Figeys, Daniel 1,3
刊名	FEBS JOURNAL
	2011-10
卷号	278 期号:20 页码:3796-3806
关键词	fractionation immobilized enzyme reactor liquid chromatography mass spectrometry microfluidic post-translational modification proteomic reactor proteomics strong anion exchange strong cation exchange
ISSN号	1742-464X
DOI	10.1111/j.1742-4658.2011.08292.x
文献子类	Review
英文摘要	Proteomic analysis requires the combination of an extensive suite of technologies including protein processing and separation, micro-flow HPLC, MS and bioinformatics. Although proteomic technologies are still in flux, approaches that bypass gel electrophoresis (gel-free approaches) are dominating the field of proteomics. Along with the development of gel-free proteomics, came the development of devices for the processing of proteomic samples termed proteomic reactors. These microfluidic devices provide rapid, robust and efficient pre-MS sample procession by performing protein sample preparation/concentration, digestion and peptide fractionation. The proteomic reactor has advanced in two major directions: immobilized enzyme reactor and ion exchange-based proteomic reactor. This review summarizes the technical developments and biological applications of the proteomic reactor over the last decade.
资助项目	CIHR[00000000]
WOS关键词	STRONG CATION-EXCHANGE ; HIGHLY EFFICIENT PROTEOLYSIS ; TANDEM MASS-SPECTROMETRY ; PROTEIN IDENTIFICATION ; LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY-IONIZATION ; MICROFLUIDIC REACTOR ; PEPTIDE SEPARATION ; MEMBRANE PROTEOME ; N-GLYCOPROTEOME
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000295335500002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278389]
专题	分析化学研究室
通讯作者	Figeys, Daniel
作者单位	1.Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing 100864, Peoples R China; 3.Univ Ottawa, OISB, Ottawa, ON K1H 8M5, Canada; 4.Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Hu,Ning, Zhibin,Wang, Fangjun,et al. Proteomic reactors and their applications in biology[J]. FEBS JOURNAL,2011,278(20):3796-3806.
APA	Zhou, Hu,Ning, Zhibin,Wang, Fangjun,Seebun, Deeptee,&Figeys, Daniel.(2011).Proteomic reactors and their applications in biology.FEBS JOURNAL,278(20),3796-3806.
MLA	Zhou, Hu,et al."Proteomic reactors and their applications in biology".FEBS JOURNAL 278.20(2011):3796-3806.