Metabonomic analysis of the therapeutic effect of Zhibai Dihuang Pill in treatment of streptozotocin-induced diabetic nephropathy

doi:10.1016/j.jep.2012.05.031

	Metabonomic analysis of the therapeutic effect of Zhibai Dihuang Pill in treatment of streptozotocin-induced diabetic nephropathy
	Zhao, Liangcai 1,3; Gao, Hongchang 2; Zhao, Yongxiang 3; Lin, Donghai 1,4
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2012-08-01
卷号	142 期号:3 页码:647-656
关键词	Metabonomics Nuclear magnetic resonance Diabetic nephropathy Zhibai Dihuang Pill
ISSN号	0378-8741
DOI	10.1016/j.jep.2012.05.031
文献子类	Article
英文摘要	Ethnopharmacological relevance: Zhibai Dihuang Pill (ZDP) is one of ancient traditional Chinese medicines (TCMs), which is usually used for the treatment of kidney deficiency for thousands of years in China. Aim of the study: Traditional Chinese medicines (TCMs) usually operate in vivo through multi-components, multi-ways and multi-targets. However, the molecular mechanisms of TCMs remain unclear. In the present work, nuclear magnetic resonance (NMR)-based metabonomic analysis was used to evaluate the therapeutic effect of Zhibai Dihuang Pill (ZDP) on diabetic nephropathy (DN) rats induced by streptozotocin and to address the underlying molecular mechanism. Materials and methods: Male rats were divided into three groups: control, DN and ZDP-treated DN (ZDP-DN), respectively. Based on H-1 NMR spectra of sera, urine and kidney extracts from the rats, principle component analysis (PCA) was performed to identify different metabolic profiles. Kidney portions and serum and urine samples were also subjected to histopathological or biochemical examination. Results: PCA scores plots demonstrate that the cluster of DN rats is separated from that of control rats, while some of ZDP-DN rats are located close to control rats, indicating that metabolic profiles of these ZDP-DN rats are restored toward those of control rats. Our results illustrate that ZDP treatment could lower the levels of lipids and 3-hydrobutyrate, and raise the level of lactate in sera of DN rats. Moreover, ZDP treatment could also reduce the levels of glucose, 3-hydrobutyrate and lactate, enhance the level of betaine in kidney tissues. Conclusion: Our study indicates that ZDP treatment can ameliorate DN symptoms by intervening in some dominating metabolic pathways, such as inhibiting glucose and lipid metabolism, enhancing methylamine metabolism. Our work may be of benefit to both evaluation of the therapeutic effect of TCM and elucidation of the underlying molecular mechanism. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[81100598] ; National Natural Science Foundation of China[91129713] ; National Natural Science Foundation of China[31170717] ; National Natural Science Foundation of China[31000335] ; Program of Shanghai Subject Chief Scientist[09XD1405100]
WOS关键词	MAGNETIC-RESONANCE-SPECTROSCOPY ; ET-ROS AXIS ; MELLITUS PATIENTS ; CORNI-FRUCTUS ; RENAL-CORTEX ; RATS ; METABOLOMICS ; METABOLISM ; IDENTIFICATION ; DYSFUNCTION
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000307035300007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277997]
专题	分析化学研究室
通讯作者	Lin, Donghai
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Wenzhou Med Coll, Sch Pharm, Wenzhou 325035, Peoples R China; 3.Guangxi Med Univ, Biol Targeting Diag & Therapy Res Ctr, Nanning 530021, Guangxi, Peoples R China 4.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen 361005, Peoples R China;
推荐引用方式 GB/T 7714	Zhao, Liangcai,Gao, Hongchang,Zhao, Yongxiang,et al. Metabonomic analysis of the therapeutic effect of Zhibai Dihuang Pill in treatment of streptozotocin-induced diabetic nephropathy[J]. JOURNAL OF ETHNOPHARMACOLOGY,2012,142(3):647-656.
APA	Zhao, Liangcai,Gao, Hongchang,Zhao, Yongxiang,&Lin, Donghai.(2012).Metabonomic analysis of the therapeutic effect of Zhibai Dihuang Pill in treatment of streptozotocin-induced diabetic nephropathy.JOURNAL OF ETHNOPHARMACOLOGY,142(3),647-656.
MLA	Zhao, Liangcai,et al."Metabonomic analysis of the therapeutic effect of Zhibai Dihuang Pill in treatment of streptozotocin-induced diabetic nephropathy".JOURNAL OF ETHNOPHARMACOLOGY 142.3(2012):647-656.