3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations | |
Cui, P. X.1,2; Lian, F. L.5; Wang, Y.1,2; Wen, Yi5; Chu, W. S.1,2; Zhao, H. F.2; Zhang, S.4; Li, J.4; Lin, D. H.3; Wu, Z. Y.1,2 | |
刊名 | RADIATION PHYSICS AND CHEMISTRY
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2014-02 | |
卷号 | 95页码:69-72 |
关键词 | RaPrP Copper XANES MXAN EXAFS |
ISSN号 | 0969-806X |
DOI | 10.1016/j.radphyschem.2013.01.043 |
文献子类 | Article |
英文摘要 | Prion-related protein (PrP), a cell-surface copper-binding glycoprotein, is considered to be responsible for a number of transmissible spongiform encephalopathies (TSEs). The structural conversion of PrP from the normal cellular isoform (PrPC) to the post-translationally modified form (PrPSc) is thought to be relevant to Cu2+ binding to histidine residues. Rabbits are one of the few mammalian species that appear to be resistant to TSEs, because of the structural characteristics of the rabbit prion protein (RaPrPC) itself. Here we determined the three-dimensional local structure around the C-terminal high-affinity copper-binding sites using X-ray absorption near-edge structure combined with ab initio calculations in the framework of the multiple-scattering (MS) theory. Result shows that two amino acid resides, Gln97 and Met108, and two histidine residues, His95 and His110, are involved in binding this copper(II) ion. It might help us understand the roles of copper in prion conformation conversions, and the molecular mechanisms of prion-involved diseases. (C) 2013 Elsevier Ltd. All rights reserved. |
资助项目 | Knowledge Innovation Program of the Chinese Academy of Sciences[KJCX2-YW-N42] ; Key Important Project of the National Natural Science Foundation of China[10734070] ; National Natural Science Foundation of China[NSFC 10805055] ; National Natural Science Foundation of China[11275227] ; National Natural Science Foundation of China[31170717] ; National Natural Science Foundation of China[91129713] ; National Basic Research Program of China[2009CB930804] |
WOS关键词 | X-RAY-ABSORPTION ; SPECTRA ; SCRAPIE |
WOS研究方向 | Chemistry ; Nuclear Science & Technology ; Physics |
语种 | 英语 |
出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
WOS记录号 | WOS:000329271400016 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/277215] ![]() |
专题 | 分析化学研究室 |
通讯作者 | Chu, W. S. |
作者单位 | 1.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Peoples R China; 2.Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China; 3.Xiamen Univ, Key Lab Chem Biol Fujian Prov, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China; 4.Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
推荐引用方式 GB/T 7714 | Cui, P. X.,Lian, F. L.,Wang, Y.,et al. 3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations[J]. RADIATION PHYSICS AND CHEMISTRY,2014,95:69-72. |
APA | Cui, P. X..,Lian, F. L..,Wang, Y..,Wen, Yi.,Chu, W. S..,...&Wu, Z. Y..(2014).3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations.RADIATION PHYSICS AND CHEMISTRY,95,69-72. |
MLA | Cui, P. X.,et al."3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations".RADIATION PHYSICS AND CHEMISTRY 95(2014):69-72. |
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