Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes

	Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes
	Liu, X; Zheng, SX; Zhou, LJ; Zhu, XZ
刊名	ACTA PHARMACOLOGICA SINICA
	2000-01
卷号	21 期号:1 页码:19-22
关键词	basic fibroblast growth factor astrocytes endothelial growth factors cultured cells genetic transcription polymerase chain reaction message RNA immunohistochemistry
ISSN号	0253-9756
文献子类	Article
英文摘要	AIM: To examine the effect of recombinant human basic fibroblast growth factor(bFGF) on the expression of vascular endothelial growth factor (VEGF) in primary cultured rat astrocytes. METHODS: Semiquantification PCR (SQ-PCR) and immunocytochemistry were used to investigate the effect of bFGF on VEGF mRNA level and protein level, respectively. RESULTS: Treatment with bFGF dose-dependently increased the VEGF mRNA level in astrocytes. The up-regulation of VEGF mRNA induced by bFGF (10 mu g/L) was detected as short as 3-h treatment. The increase of VEGF mRNA level reached the maximum after 24-h treatment with bFGF. The immunocytochemical staining showed that the VEGF protein level in astrocytes also increased after the cells were incubated with bFGF. CONCLUSION: bFGF induced a marked time- and concentration-dependent increase in VEGF expression in primary cultured astrocytes, suggesting that the effect of bFGF on angiogenesis in brain may act partly by up-regulating VEGF expression in astrocytes.
WOS关键词	FOCAL CEREBRAL-ISCHEMIA ; FACTOR FAMILY ; BRAIN ; ANGIOGENESIS ; REDUCTION ; INDUCTION ; FOREBRAIN ; DAMAGE
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000084710700002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274652]
专题	分析化学研究室药理学第二研究室药物安全性评价中心
通讯作者	Zhu, XZ
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol 1, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Liu, X,Zheng, SX,Zhou, LJ,et al. Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes[J]. ACTA PHARMACOLOGICA SINICA,2000,21(1):19-22.
APA	Liu, X,Zheng, SX,Zhou, LJ,&Zhu, XZ.(2000).Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes.ACTA PHARMACOLOGICA SINICA,21(1),19-22.
MLA	Liu, X,et al."Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes".ACTA PHARMACOLOGICA SINICA 21.1(2000):19-22.