Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes | |
Liu, X![]() | |
刊名 | ACTA PHARMACOLOGICA SINICA
![]() |
2000-01 | |
卷号 | 21期号:1页码:19-22 |
关键词 | basic fibroblast growth factor astrocytes endothelial growth factors cultured cells genetic transcription polymerase chain reaction message RNA immunohistochemistry |
ISSN号 | 0253-9756 |
文献子类 | Article |
英文摘要 | AIM: To examine the effect of recombinant human basic fibroblast growth factor(bFGF) on the expression of vascular endothelial growth factor (VEGF) in primary cultured rat astrocytes. METHODS: Semiquantification PCR (SQ-PCR) and immunocytochemistry were used to investigate the effect of bFGF on VEGF mRNA level and protein level, respectively. RESULTS: Treatment with bFGF dose-dependently increased the VEGF mRNA level in astrocytes. The up-regulation of VEGF mRNA induced by bFGF (10 mu g/L) was detected as short as 3-h treatment. The increase of VEGF mRNA level reached the maximum after 24-h treatment with bFGF. The immunocytochemical staining showed that the VEGF protein level in astrocytes also increased after the cells were incubated with bFGF. CONCLUSION: bFGF induced a marked time- and concentration-dependent increase in VEGF expression in primary cultured astrocytes, suggesting that the effect of bFGF on angiogenesis in brain may act partly by up-regulating VEGF expression in astrocytes. |
WOS关键词 | FOCAL CEREBRAL-ISCHEMIA ; FACTOR FAMILY ; BRAIN ; ANGIOGENESIS ; REDUCTION ; INDUCTION ; FOREBRAIN ; DAMAGE |
WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | ACTA PHARMACOLOGICA SINICA |
WOS记录号 | WOS:000084710700002 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/274652] ![]() |
专题 | 分析化学研究室 药理学第二研究室 药物安全性评价中心 |
通讯作者 | Zhu, XZ |
作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol 1, Shanghai 200031, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, X,Zheng, SX,Zhou, LJ,et al. Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes[J]. ACTA PHARMACOLOGICA SINICA,2000,21(1):19-22. |
APA | Liu, X,Zheng, SX,Zhou, LJ,&Zhu, XZ.(2000).Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes.ACTA PHARMACOLOGICA SINICA,21(1),19-22. |
MLA | Liu, X,et al."Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes".ACTA PHARMACOLOGICA SINICA 21.1(2000):19-22. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论