Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum

doi:10.1248/cpb.50.623

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum
	Bao, XF; Zhen, Y; Ruan, L; Fang, JN
刊名	CHEMICAL & PHARMACEUTICAL BULLETIN
	2002-05
卷号	50 期号:5 页码:623-629
关键词	Ganoderma lucidum polysaccharide T lymphocyte-stimulating activity sulfation carboxymethylation solution conformation
ISSN号	0009-2363
DOI	10.1248/cpb.50.623
文献子类	Article
英文摘要	The hot-water extract of the spores of Ganoderma lucidum was shown to have a stimulating effect on concanavalin A-induced mitogenic activity of T lymphocytes. Bioassay-guided separation led to the isolation of a polysaccharide with potent T lymphocyte-stimulating activity by ethanol fractionation, anion-exchange, and size-exclusion chromatography. Based on the composition and methylation analyses, periodate oxidation, Smith degradation, and NMR spectroscopy, the native polysaccharide was shown to be a beta-D-(1-->3)-glucan with branches of terminal glucosyl residues substituted at C-6 of the glucose residues in the main chain. The branching ratio is approximately 20%. A series of sulfated or carboxymethylated derivatives were prepared and their structural features were elucidated by chemical and spectral analyses. The solution conformation and T lymphocyte proliferation effect of the glucans before and after derivatization were compared and discussed. The data obtained indicate that the introduction of ionic groups would significantly affect the original conformation of the native glucan in aqueous solution and further affect T lymphocyte-stimulating activity. The triple-helical structure of the glucans, the nature of the ionic groups, and the density of negative charge were considered to be closely related to this activity.
WOS关键词	ANTITUMOR-ACTIVITY ; INHIBITORY ACTIVITY ; GLUCAN ; CURDLAN ; (1->3)-BETA-D-GLUCANS ; DERIVATIVES ; TRITERPENES ; BEHAVIOR ; SULFATE ; HIV-1
WOS研究方向	Pharmacology & Pharmacy ; Chemistry
语种	英语
出版者	PHARMACEUTICAL SOC JAPAN
WOS记录号	WOS:000175370700011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274365]
专题	药理学第三研究室天然药物化学研究室
通讯作者	Fang, JN
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Bao, XF,Zhen, Y,Ruan, L,et al. Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum[J]. CHEMICAL & PHARMACEUTICAL BULLETIN,2002,50(5):623-629.
APA	Bao, XF,Zhen, Y,Ruan, L,&Fang, JN.(2002).Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum.CHEMICAL & PHARMACEUTICAL BULLETIN,50(5),623-629.
MLA	Bao, XF,et al."Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum".CHEMICAL & PHARMACEUTICAL BULLETIN 50.5(2002):623-629.