Molecular mechanism of metabolic changes in rat cerebral cortex after cerebral ischemia-reperfusion:an NMRbased study

	Molecular mechanism of metabolic changes in rat cerebral cortex after cerebral ischemia-reperfusion:an NMRbased study
	Ma Xiazhen 1; Zhang Tianshu 1; Ruan Zhi 2; Huang Jin 1; Zhang Naixia3 ; Liu Xia3
刊名	Academic Journal of Second Military Medical University
	2016
卷号	37 期号:11 页码:1338-1347
关键词	nuclear magnetic resonance stroke brain ischemia reperfusion injuries metabolic disorders molecular mechanism
ISSN号	0258-879X
其他题名	基于核磁共振技术研究脑缺血再灌注大鼠脑皮质代谢物组水平变化的分子机制
文献子类	Article
英文摘要	Objective To investigate the relationship between metabolite levels and the time of cerebral ischemiareperfusion in the left cortex of rats,so as to explore the molecular mechanism of cortical metabolic disorders induced by ischemia-reperfusion injury.Methods Stroke models of focal cerebral ischemia in rats were established by middle cerebral artery occlusion(MCAO).Then a nuclear magnetic resonance (NMR)-based metabolome analytical approach was carried out to analyze the metabolite levels in the left cortex of MCAO rats at different time points(3,6,and 24h)after reperfusion.Results Some changes of metabolic pathways,such as energy deficiency,glycolysis aggravation,and neurotransmitter disorders,were observed in the left cortex of MCAO rats at 3h after reperfusion.All the above-mentioned disorders were alleviated by the autoregulation at 6h after reperfusion.However,the forementioned metabolic disturbances became severe after 24-hour reperfusion.Conclusion Our results suggest that different extents of metabolic disturbance appears in the cortex at different time points after reperfusion,which might help to understand the molecular mechanism of cerebral ischemia-reperfusion injury, providing reference for regulating metabolic disorders at different time points after stroke in clinic.
资助项目	国家自然科学基金[00000000] ; 国家重点基础研究发展计划(973计划)[00000000]
WOS研究方向	Neurosciences & Neurology (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:5864480
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269225]
专题	分析化学研究室药物安全性评价中心
通讯作者	Zhang Naixia; Liu Xia
作者单位	1.Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology, Shanghai 200237, China.; 2.Key Laboratory of Receptor Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China.; 3.Department of Analytical Chemistry,Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	Ma Xiazhen,Zhang Tianshu,Ruan Zhi,et al. Molecular mechanism of metabolic changes in rat cerebral cortex after cerebral ischemia-reperfusion:an NMRbased study[J]. Academic Journal of Second Military Medical University,2016,37(11):1338-1347.
APA	Ma Xiazhen,Zhang Tianshu,Ruan Zhi,Huang Jin,Zhang Naixia,&Liu Xia.(2016).Molecular mechanism of metabolic changes in rat cerebral cortex after cerebral ischemia-reperfusion:an NMRbased study.Academic Journal of Second Military Medical University,37(11),1338-1347.
MLA	Ma Xiazhen,et al."Molecular mechanism of metabolic changes in rat cerebral cortex after cerebral ischemia-reperfusion:an NMRbased study".Academic Journal of Second Military Medical University 37.11(2016):1338-1347.