Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro

doi:10.1016/j.dci.2011.12.001

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物发现与设计中心

	Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro
	Liu, Fei 1; Chen, Yang 1; Yang, Bing 1; Wang, Jingfang 3; Peng, Qin 1; Shao, Qimiao 1; Li, Xuan 1; Beerntsen, Brenda T.2; Xu, Yechun6 ; Li, Jianyong 5
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2012-03
卷号	36 期号:3 页码:619-628
关键词	Drosophila melanogaster Prophenoloxidases Expression Cu2+ association Activation
ISSN号	0145-305X
DOI	10.1016/j.dci.2011.12.001
文献子类	Article
英文摘要	Dipteran insects, like mosquitoes, possess more than two prophenoloxidase (PPO) genes, but it is unclear whether their gene products differ in biochemical properties and physiological functions. Here, we used three Drosophila melanogaster PPOs as models to study their properties through expression in S2 cells. Our data revealed that the PPOs were expressed in the ethanol-activatable conformation: rPPO1 and rPPO2 needed additional Cu2+ in the medium, but rPPO3 did not. rPPO1 bound Cu2+ within minutes; rPPO2 did that in hours when Cu2+ were present at a higher concentration. Thus, rPPO1 and rPPO2 were expressed as apo-rPPO and became holo-PPO upon Cu2+ binding; rPPO3 was holo-PPO immediately after expression. Surprisingly, in the absence of ethanol, the apparently intact rPPO3 catalyzed dopamine oxidation and melanization. The successful method for rPPO expression in S2 cells described in this paper will provide us with an opportunity to study the properties of a specific PPO gene in a small insect like mosquitoes in the future. (c) 2011 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[30970408] ; National Natural Science Foundation of China[31172151] ; Chinese Academy of Sciences[KSCX2-EW-J-12] ; Chinese Academy of Sciences[20090HTP05] ; Ministry of Agriculture of China[2008ZX08009-003]
WOS关键词	MANDUCA-SEXTA ; PHENOLOXIDASE ACTIVITY ; IMMUNE-RESPONSES ; ACTIVATION ; INSECT ; MELANIZATION ; 2-PROPANOL ; HEMOLYMPH ; A(1)
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000301170800015
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278172]
专题	药物发现与设计中心
通讯作者	Ling, Erjun
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Insect Dev & Evolutionary Biol, Shanghai 200032, Peoples R China; 2.Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA; 3.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, Shanghai 200240, Peoples R China; 4.Univ Missouri, Sch Biol Sci, Div Cell Biol & Biophys, Kansas City, MO 64110 USA 5.Virginia Polytech Inst & State Univ, Dept Biochem, Blacksburg, VA 24061 USA; 6.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Discovery & Design, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Fei,Chen, Yang,Yang, Bing,et al. Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,36(3):619-628.
APA	Liu, Fei.,Chen, Yang.,Yang, Bing.,Wang, Jingfang.,Peng, Qin.,...&Ling, Erjun.(2012).Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,36(3),619-628.
MLA	Liu, Fei,et al."Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 36.3(2012):619-628.