Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy

doi:10.1002/bmc.3248

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物质量控制与固体化学研究中心

	Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy
	Zhao, Lijuan; Chen, Dongying
刊名	BIOMEDICAL CHROMATOGRAPHY
	2015-01
卷号	29 期号:1 页码:123-128
关键词	capillary electrophoresis frontal analysis circular dichroism drug binding methoxatin disodium salt human serum albumin
ISSN号	0269-3879
DOI	10.1002/bmc.3248
文献子类	Article
英文摘要	Pressure-assisted capillary electrophoresis (PACE)/frontal analysis (FA) and circular dichroism spectroscopy were utilized to investigate the interactions between methoxatin disodium salt (PQQ-2Na) and human serum albumin (HSA). With the PACE/FA method, sodium phosphate buffer solution (67mm, pH7.4) was used as the background electrolyte. Hydrodynamic injection at 50mbar for 50s and external pressure of 50mbar were applied. The binding constant and the number of primary binding sites to HSA were obtained under fixed concentration of PQQ-2Na (100 mu m) and increasing HSA concentration (0 similar to 475 mu m). The thermodynamic parameters characterized the main acting forces of hydrophobic and electrostatic interactions. The displacement experiments using phenylbutazone and flurbiprofen as ligand markers suggested that the binding site was the Sudlow site I of the HSA molecule. Circular dichroism spectroscopy was further employed to evaluate the conformation changes of HSA under the interaction of PQQ-2Na. This work provides comprehensive information for understanding the interactions between PQQ-2Na and HSA. Copyright (c) 2014 John Wiley & Sons, Ltd.
资助项目	Key New Drug Creation and Manufacturing Program, National Science and Technology Major Project of China[2012ZX09301001-001]
WOS关键词	PLASMA-PROTEIN-BINDING ; PYRROLOQUINOLINE QUINONE ; FRONTAL ANALYSIS ; DRUG-BINDING ; EQUILIBRIUM DIALYSIS ; HIGH-THROUGHPUT ; IN-VITRO ; CONSTANTS ; FORCES ; SITE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000346908100014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276776]
专题	药物质量控制与固体化学研究中心上海药物代谢研究中心
通讯作者	Chen, Dongying
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Lab Pharmaceut Anal, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lijuan,Chen, Dongying. Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy[J]. BIOMEDICAL CHROMATOGRAPHY,2015,29(1):123-128.
APA	Zhao, Lijuan,&Chen, Dongying.(2015).Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy.BIOMEDICAL CHROMATOGRAPHY,29(1),123-128.
MLA	Zhao, Lijuan,et al."Characterization of interactions between methoxatin disodium salt and human serum albumin by pressure-assisted capillary electrophoresis/frontal analysis and circular dichroism spectroscopy".BIOMEDICAL CHROMATOGRAPHY 29.1(2015):123-128.