CTC clusters induced by heparanase enhance breast cancer metastasis

doi:10.1038/aps.2017.189

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	CTC clusters induced by heparanase enhance breast cancer metastasis
	Wei, Rong-rui 1; Sun, Dan-ni 2; Yang, Hong 2; Yan, Juan 2; Zhang, Xiong 2; Zheng, Xing-ling 2; Fu, Xu-hong 2; Geng, Mei-yu2 ; Huang, Xun2 ; Ding, Jian2
刊名	ACTA PHARMACOLOGICA SINICA
	2018-08
卷号	39 期号:8 页码:1326-1337
关键词	human breast cancer CTC clusters heparanase metastasis cell adhesion adhesion molecules suspension culture method
ISSN号	1671-4083
DOI	10.1038/aps.2017.189
文献子类	Article
英文摘要	Aggregated metastatic cancer cells, referred to as circulating tumor cell (CTC) clusters, are present in the blood of cancer patients and contribute to cancer metastasis. However, the origin of CTC clusters, especially intravascular aggregates, remains unknown. Here, we employ suspension culture methods to mimic CTC cluster formation in the circulation of breast cancer patients. CTC clusters generated using these methods exhibited an increased metastatic potential that was defined by the overexpression of heparanase (HPSE). Heparanase induced FAK- and ICAM-1-dependent cell adhesion, which promoted intravascular cell aggregation. Moreover, knockdown of heparanase or inhibition of its activity with JG6, a heparanase inhibitor, was sufficient to block the formation of cell clusters and suppress breast cancer metastasis. Our data reveal that heparanase-mediated cell adhesion is critical for metastasis mediated by intravascular CTC clusters. We also suggest that targeting the function of heparanase in cancer cell dissemination might limit metastatic progression.
资助项目	National Natural Science Foundation of China[81302791] ; Youth Innovation Promotion Association CAS[00000000] ; Chinese Academy of Sciences[XDA12020326] ; Shanghai Talent Development Funds[201663]
WOS关键词	CIRCULATING TUMOR-CELLS ; FOCAL ADHESION KINASE ; INHIBITOR PI-88 ; LUNG-CANCER ; ANGIOGENESIS ; DISSEMINATION ; PLAKOGLOBIN ; MIGRATION ; MELANOMA ; SULFATE
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:6309897
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000441241700008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279633]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Huang, Xun; Ding, Jian
作者单位	1.China Pharmaceut Univ, Dept New Drug Screening Ctr, Nanjing 210009, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wei, Rong-rui,Sun, Dan-ni,Yang, Hong,et al. CTC clusters induced by heparanase enhance breast cancer metastasis[J]. ACTA PHARMACOLOGICA SINICA,2018,39(8):1326-1337.
APA	Wei, Rong-rui.,Sun, Dan-ni.,Yang, Hong.,Yan, Juan.,Zhang, Xiong.,...&Ding, Jian.(2018).CTC clusters induced by heparanase enhance breast cancer metastasis.ACTA PHARMACOLOGICA SINICA,39(8),1326-1337.
MLA	Wei, Rong-rui,et al."CTC clusters induced by heparanase enhance breast cancer metastasis".ACTA PHARMACOLOGICA SINICA 39.8(2018):1326-1337.