(5R)-5-hydroxytriptolide inhibits the immune response of human peripheral blood mononuclear cells | |
Zhou, Ru3; Tang, Wei3![]() ![]() | |
刊名 | INTERNATIONAL IMMUNOPHARMACOLOGY
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2009-01 | |
卷号 | 9期号:1页码:63-69 |
关键词 | (5R)-5-hydroxytriptolide Cytokine MAPK PBMC |
ISSN号 | 1567-5769 |
DOI | 10.1016/j.intimp.2008.09.014 |
文献子类 | Article |
英文摘要 | Aim: (5R)-5-hydroxytriptolide (LLDT-8) displayed immunosuppressive activities both in vitro and in autoimmune disease models. Here, we aim to further clarify the effect of LLDT-8 on the immune responses of human peripheral blood mononuclear cells (PBMC). Method: Cell proliferation of human PBMC from healthy donors was evaluated by [H-3]-thymidine uptake. NK cell cytotoxicity was assayed using K562 cells in a 13 HI lysis assay. Cytokine production was determined by enzyme-linked immunosorbent assay. The expression of cell surface molecules was detected with flow cytometry. The mRNA expression and the protein phosphorylation levels were detected by RT-PCR and Western inimunoblot assay. Results: LLDT-8 at 25 and 50 nM significantly inhibited the PHA- and recall antigens-induced T cell proliferation, and Suppressed mixed lymphocyte reaction. LLDT-8 reduced cytokines production (IFN-gamma, IL-2, TNF-alpha) in PHA- and Sac-activated PBMC. LLDT-8 did not alter the increased expression of MHC class I/II and B7.1, but reduced B7.2 by approximately 30%. No effect of LLDT-8 was observed for the expression of T cell activation markers (CD69, CD154). However, LLDT-8 significantly reduced IFN-gamma-expressing T cell percentages and IFN-gamma mRNA transcription in PHA-activated T cells. It also inhibited the phosphorylation levels of JNK and p38. LLDT-8 did not affect NK cytotoxic activity against K562 cells. Conclusion: LLDT-8 was a promising immunosuppressant for human immune-related diseases. (C) 2008 Published by Elsevier B.V. |
资助项目 | Shanghai Science and Technology Committee[064319014] ; Shanghai Science and Technology Committee[074319110] |
WOS关键词 | NATURAL-KILLER-CELLS ; T-CELLS ; MAP KINASES ; ANTIGEN RECEPTOR ; IN-VITRO ; LLDT-8 ; JNK ; EXPRESSION ; MICE ; DIFFERENTIATION |
WOS研究方向 | Immunology ; Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000263144300009 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/279387] ![]() |
专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
通讯作者 | Zuo, Jian-Ping |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Lab, State Key Lab Drug Res,Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunopharmacol, State Key Lab Drug Res,Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China; |
推荐引用方式 GB/T 7714 | Zhou, Ru,Tang, Wei,He, Pei-Lan,et al. (5R)-5-hydroxytriptolide inhibits the immune response of human peripheral blood mononuclear cells[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2009,9(1):63-69. |
APA | Zhou, Ru,Tang, Wei,He, Pei-Lan,Yang, Yi-Fu,Li, Yuan-Chao,&Zuo, Jian-Ping.(2009).(5R)-5-hydroxytriptolide inhibits the immune response of human peripheral blood mononuclear cells.INTERNATIONAL IMMUNOPHARMACOLOGY,9(1),63-69. |
MLA | Zhou, Ru,et al."(5R)-5-hydroxytriptolide inhibits the immune response of human peripheral blood mononuclear cells".INTERNATIONAL IMMUNOPHARMACOLOGY 9.1(2009):63-69. |
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