WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro

doi:10.1038/aps.2010.29

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	WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro
	Tong, Xian-kun3 ; Qiu, Hong 1; Zhang, Xin 2; Shi, Li-ping 3; Wang, Gui-feng3 ; Ji, Fei-hong 3; Ding, Hui-yong 3; Tang, Wei3 ; Ding, Kan1 ; Zuo, Jian-ping3,4
刊名	ACTA PHARMACOLOGICA SINICA
	2010-05
卷号	31 期号:5 页码:585-592
关键词	Dengue virus sulfated polysaccharide infection BHK cells alpha-D-glucan
ISSN号	1671-4083
DOI	10.1038/aps.2010.29
文献子类	Article
英文摘要	Aim: To investigate the mode of action of WSS45, one sulfated derivative of an alpha-D-glucan from the Gastrodia elata Bl, on the multiplication cycle of dengue virus serotype 2 (DV2), including initial infection and intracellular replication. Methods: Virus multiplication in BHK cells were monitored by qRT-PCR, plaque reduction assay, and flow cytometry. Initial virus infection was dissected into adsorption and penetration steps by converting temperature and treating by acid glycine. Surface bound virions were detected by immunofluorescence staining for Evelope protein. Results: WSS45 effectively inhibited DV2 infection in BHK cells with an EC50 value of 0.68 +/- 0.17 mu g/mL, mainly interfered with virus adsorption, in a very early stage of the virus cycle. However, WSS45 showed no viricidal effect. Moreover, WSS45 could increase the detaching of virus from cell surface in BHK cell line. Conclusion: WSS45 exerted potent inhibitory effect on DV2 through interfering with the interaction between viruses and targeted cells. This activity was related to its molecular size.
资助项目	European Union[517711] ; National Science & Technology Major Project[2009ZX09301-001] ; Science & Technology Commission of Shanghai Municipality, China[08XD14053] ; Science & Technology Commission of Shanghai Municipality, China[08410708900]
WOS关键词	HEPARAN-SULFATE ; CELLS ; GLYCOPROTEIN ; BINDING ; IDENTIFICATION ; ACTIVATION ; ENTRY
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:3893588
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000277661600009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278903]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室药理学第三研究室
通讯作者	Tang, Wei
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; 2.Inst Ctr Dis Control & Prevent Guangdong Prov, Guangzhou 510300, Guangdong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunopharmacol, Shanghai 201203, Peoples R China; 4.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Tong, Xian-kun,Qiu, Hong,Zhang, Xin,et al. WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro[J]. ACTA PHARMACOLOGICA SINICA,2010,31(5):585-592.
APA	Tong, Xian-kun.,Qiu, Hong.,Zhang, Xin.,Shi, Li-ping.,Wang, Gui-feng.,...&Zuo, Jian-ping.(2010).WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro.ACTA PHARMACOLOGICA SINICA,31(5),585-592.
MLA	Tong, Xian-kun,et al."WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro".ACTA PHARMACOLOGICA SINICA 31.5(2010):585-592.