Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity | |
Li, Song2; Zhang, Xue-Yong2; Zhang, Su-Yin1; Chen, Xi-Tao2; Chen, Ling-Ji1; Shu, Yu-Hua1; Zhang, Jia-Liu1; Fan, Dai-Ming2 | |
刊名 | BIOCONJUGATE CHEMISTRY
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1990-07 | |
卷号 | 1期号:4页码:245-250 |
ISSN号 | 1043-1802 |
DOI | 10.1021/bc00004a003 |
文献子类 | Article |
英文摘要 | In the present study, an antigastric cancer monoclonal antibody, MGb(2), was chosen to prepare antibody-mitomycin C conjugate with dextran T-40 as intermediary. Up to 20 molecules of mitomycin C were specifically bound per molecule of antibody, without significantly impairing the antigen-binding capacity of the antibody and the pharmacological activity of mitomycin C. The conjugate showed selective cytotoxicity upon human gastric cancer cell line SGC-7901 in vitro. Radioimmunoimaging and biodistribution studies indicated that, after conjugation with mitomycin C via dextran T-40 as intermediary, the tumor localization capacity of the antibody was well-retained. When tested in nude mice inoculated with human gastric carcinoma GAII in bilateral subrenal capsules, intraperitoneal injection of the conjugate twice a week for 3 weeks at the dose of 1 mg/kg of drug gave a tumor inhibitory rate of 152.29%, the result being far better than that of free mitomycin C or an irrelevant conjugate. A similar result was found in another nude mouse model of human gastric carcinoma SGC-7901. Meanwhile, after conjugation with antibody, the toxicity of mitomycin C on tested animals was significantly reduced. |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000206175200003 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/275247] ![]() |
专题 | 成果转移转化处 |
通讯作者 | Li, Song |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Xijing Hosp, Lab Gastroenterol, Xian 710032, Shaanxi, Peoples R China; |
推荐引用方式 GB/T 7714 | Li, Song,Zhang, Xue-Yong,Zhang, Su-Yin,et al. Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity[J]. BIOCONJUGATE CHEMISTRY,1990,1(4):245-250. |
APA | Li, Song.,Zhang, Xue-Yong.,Zhang, Su-Yin.,Chen, Xi-Tao.,Chen, Ling-Ji.,...&Fan, Dai-Ming.(1990).Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity.BIOCONJUGATE CHEMISTRY,1(4),245-250. |
MLA | Li, Song,et al."Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity".BIOCONJUGATE CHEMISTRY 1.4(1990):245-250. |
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