Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity

doi:10.1021/bc00004a003

	Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity
	Li, Song 2; Zhang, Xue-Yong 2; Zhang, Su-Yin 1; Chen, Xi-Tao 2; Chen, Ling-Ji 1; Shu, Yu-Hua 1; Zhang, Jia-Liu 1; Fan, Dai-Ming 2
刊名	BIOCONJUGATE CHEMISTRY
	1990-07
卷号	1 期号:4 页码:245-250
ISSN号	1043-1802
DOI	10.1021/bc00004a003
文献子类	Article
英文摘要	In the present study, an antigastric cancer monoclonal antibody, MGb(2), was chosen to prepare antibody-mitomycin C conjugate with dextran T-40 as intermediary. Up to 20 molecules of mitomycin C were specifically bound per molecule of antibody, without significantly impairing the antigen-binding capacity of the antibody and the pharmacological activity of mitomycin C. The conjugate showed selective cytotoxicity upon human gastric cancer cell line SGC-7901 in vitro. Radioimmunoimaging and biodistribution studies indicated that, after conjugation with mitomycin C via dextran T-40 as intermediary, the tumor localization capacity of the antibody was well-retained. When tested in nude mice inoculated with human gastric carcinoma GAII in bilateral subrenal capsules, intraperitoneal injection of the conjugate twice a week for 3 weeks at the dose of 1 mg/kg of drug gave a tumor inhibitory rate of 152.29%, the result being far better than that of free mitomycin C or an irrelevant conjugate. A similar result was found in another nude mouse model of human gastric carcinoma SGC-7901. Meanwhile, after conjugation with antibody, the toxicity of mitomycin C on tested animals was significantly reduced.
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000206175200003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275247]
专题	成果转移转化处
通讯作者	Li, Song
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Xijing Hosp, Lab Gastroenterol, Xian 710032, Shaanxi, Peoples R China;
推荐引用方式 GB/T 7714	Li, Song,Zhang, Xue-Yong,Zhang, Su-Yin,et al. Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity[J]. BIOCONJUGATE CHEMISTRY,1990,1(4):245-250.
APA	Li, Song.,Zhang, Xue-Yong.,Zhang, Su-Yin.,Chen, Xi-Tao.,Chen, Ling-Ji.,...&Fan, Dai-Ming.(1990).Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity.BIOCONJUGATE CHEMISTRY,1(4),245-250.
MLA	Li, Song,et al."Preparation of Antigastric Cancer Monoclonal Antibody MGb(2)-Mitomycin C Conjugate with Improved Antitumor Activity".BIOCONJUGATE CHEMISTRY 1.4(1990):245-250.