RP -HPLC determination of dobesilic acid in human plasma

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	RP -HPLC determination of dobesilic acid in human plasma
	ZHANG Yini 1; CHEN Xiaoyan1 ; YANG Hongmei 2; ZHONG Dafang1
刊名	Chinese Journal of Pharmaceutical Analysis
	2007
卷号	27 期号:8 页码:1208-1211
关键词	calcium dobesilate HPLC plasma drug concentration
ISSN号	0254-1793
其他题名	反相离子对高效液相色谱法测定人血浆中羟苯磺酸
文献子类	Article
英文摘要	Objective：To develop and validate a sensitive and simple reversed -phase ion -pair HPLC method for the determination of dobesilic acid in human plasma. Methods：Mter a simple protein precipitation with acetonitrile, the analyte and the internal standard ,p -aminobenzoic acid ,were chromatographied on a Diamonsil C_(18) column with the mobile phase consisting of 0. 05 mol ·L~(-1), potassium dihydrogen phosphate buffer （ pH = 3.0） - acetonitrile （87： 13, v/v） , containing 0. 1% tetra - n - butylammonium hydroxide, at a flow rate of 1.0 mL ·min~( - 1). The UV wavelength was set at 300 nm. Results：The linear calibration curves were obtained in the range of 0. 364 -36.4 mg · L~(-1). The lower limit of quantification was 0. 364 mg·L~(-1). The inter -and intra -day RSD acrossed the three validation runs were both below 8.7% ,and the accuracy （RE） was within 4. 5% for the analyte. The mean recoveries of dobesilic acid and internal standard p- aminobenzoic acid were 74.8% and 94. 8% ,respectively. Conclusion：The method is proved to be special, convenient, and suitable for clinical investigation and pharmacokinetics of calcium dobesilate.
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:2954751
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268170]
专题	上海药物代谢研究中心药物发现与设计中心药物化学研究室药理学第一研究室
通讯作者	ZHONG Dafang
作者单位	1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.
推荐引用方式 GB/T 7714	ZHANG Yini,CHEN Xiaoyan,YANG Hongmei,et al. RP -HPLC determination of dobesilic acid in human plasma[J]. Chinese Journal of Pharmaceutical Analysis,2007,27(8):1208-1211.
APA	ZHANG Yini,CHEN Xiaoyan,YANG Hongmei,&ZHONG Dafang.(2007).RP -HPLC determination of dobesilic acid in human plasma.Chinese Journal of Pharmaceutical Analysis,27(8),1208-1211.
MLA	ZHANG Yini,et al."RP -HPLC determination of dobesilic acid in human plasma".Chinese Journal of Pharmaceutical Analysis 27.8(2007):1208-1211.