Bacillamide C production by the optimized cultivation of the Bacillus atrophaeus strain C89 associated with the South China Sea sponge Dysidea avara

doi:10.1016/j.procbio.2011.02.003

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	Bacillamide C production by the optimized cultivation of the Bacillus atrophaeus strain C89 associated with the South China Sea sponge Dysidea avara
	Jin, Liangjie 1,2; Ma, Wen 3; Peng, Chongsheng 4; Yin, Ying 1,2; Xu, Bailiang 1,2; Zhang, Fengli 1,2; Guo, Yuewei5 ; Li, Zhiyong 1,2
刊名	PROCESS BIOCHEMISTRY
	2011-05
卷号	46 期号:5 页码:1153-1159
关键词	Bacillus atrophaeus strain C89 Bacillamide C Plackett-Burman design Response surface methodology Fed-batch cultivation
ISSN号	1359-5113
DOI	10.1016/j.procbio.2011.02.003
文献子类	Article
英文摘要	Marine natural products with biological activity represent an important resource for marine drugs and biotechnological products; however, most marine natural products, especially those derived from sponges, have not been further developed because of the limited number of sources. The scale-up cultivation of the microbial source of sponge-derived natural products could provide a possible approach to solve this bottleneck problem. In this study, statistical Plackett-Burman (PB) design and Box-Behnken response surface methodology (RSM) were used to optimize the medium components to increase the production of bacillamide C by the Bacillus atrophaeus strain C89, which was associated with the marine sponge Dysidea avara. The obtained optimal medium consisted of 3.64 g/L corn starch, 6.29 g/L CaCO3, 4.00 g/L soy peptone, 6.00 g/L peptone. 0.10 g/L cysteine and 0.02 g/L tryptophan with artificial sea water (ASW). Using this optimized medium, the concentration of bacillamide C reached 70.85 mg/L in fed-batch cultivation in a 5-L fermentor at pH 7.70, which was 25.80-fold higher than the level observed in a shake flask using the basic medium. This study provides a potential approach for the production of bacillamide C and related derivatives, which was the first to optimize the cultivation of sponge-associated microbes for the production of marine natural products. (C) 2011 Elsevier Ltd. All rights reserved.
资助项目	High-Tech Research and Development Program of China[2007AA09Z447] ; National Natural Science Foundation of China (NSFC)[41076077] ; National Natural Science Foundation of China (NSFC)[30821005] ; Program for Incubation of Major Scientific Project[00000000] ; SJTU[00000000] ; Shanghai Leading Academic Discipline Project[B203]
WOS关键词	STATISTICAL OPTIMIZATION ; STRUCTURE ELUCIDATION ; MARINE BACTERIUM ; METABOLITES ; MICROORGANISMS ; DIVERSITY ; SYMBIONTS
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000290466800017
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278542]
专题	天然药物化学研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Li, Zhiyong
作者单位	1.Shanghai Jiao Tong Univ, Marine Biotechnol Lab, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China; 2.Shanghai Jiao Tong Univ, Key Lab MOE Microbial Metab, Shanghai 200240, Peoples R China; 3.Xinyang Normal Univ, Sch Chem & Chem Engn, Xinyang 464000, Henan, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Jin, Liangjie,Ma, Wen,Peng, Chongsheng,et al. Bacillamide C production by the optimized cultivation of the Bacillus atrophaeus strain C89 associated with the South China Sea sponge Dysidea avara[J]. PROCESS BIOCHEMISTRY,2011,46(5):1153-1159.
APA	Jin, Liangjie.,Ma, Wen.,Peng, Chongsheng.,Yin, Ying.,Xu, Bailiang.,...&Li, Zhiyong.(2011).Bacillamide C production by the optimized cultivation of the Bacillus atrophaeus strain C89 associated with the South China Sea sponge Dysidea avara.PROCESS BIOCHEMISTRY,46(5),1153-1159.
MLA	Jin, Liangjie,et al."Bacillamide C production by the optimized cultivation of the Bacillus atrophaeus strain C89 associated with the South China Sea sponge Dysidea avara".PROCESS BIOCHEMISTRY 46.5(2011):1153-1159.