Paeoniflorin suppresses the expression of intercellular adhesion molecule-1 (ICAM-1) in endotoxin-treated human monocytic cells | |
Jin, Lei; Zhang, Li-Min; Xie, Ke-Qiang; Ye, Yang; Feng, Linyin | |
刊名 | BRITISH JOURNAL OF PHARMACOLOGY |
2011-09 | |
卷号 | 164期号:2B页码:694-703 |
关键词 | PF ICAM-1 U937 cell adenosine A(1) receptor NF-kappa B pathway MAPK pathway HUVECs |
ISSN号 | 0007-1188 |
DOI | 10.1111/j.1476-5381.2011.01464.x |
文献子类 | Article |
英文摘要 | BACKGROUND AND PURPOSE Paeoniflorin (PF) has ameliorative effects on learning and memory impairment and cerebral ischaemia in rats and has protective effects against the degeneration of dopaminergic neurons in substantia nigra. The neuroprotective effects of PF are most probably derived from its anti-inflammatory property. Abnormally high levels of intercellular adhesion molecule-1 (ICAM-1) have been found to be associated with a wide range of inflammatory and immune responses. Here we studied whether PF regulates the levels of ICAM-1 elevated in LPS-activated differentiated human monocytic U937 cells and TNF-alpha-stimulated human umbilical vein endothelial cells (HUVECs). EXPERIMENTAL APPROACH mRNA levels were evaluated by RT-PCR. Protein levels were evaluated by Western blot analysis. An immunofluorescence technique was used to estimate NF-kappa B translocation, and NF-kappa B binding to nuclear DNA was determined by EMSA. KEY RESULTS PF inhibited ICAM-1 expression elevated in LPS-induced U937 cells and TNF-alpha-stimulated HUVECs. Although previous reports showed that PF's action is mediated by activating adenosine A(1) receptors, application of a selective adenosine A(1) receptor antagonist did not change the inhibitory effect of PF in our experiments. To elucidate the underlying mechanisms of the effect of PF, we studied its effect on signalling pathways upstream of ICAM-1 expression. PF suppressed the activation of the NF-kappa B pathway, which regulates the expression of ICAM-1. The TLR4 and MAPK pathways were shown not to be involved in the effects of PF in these cells. CONCLUSIONS AND IMPLICATIONS PF inhibits ICAM-1 expression in LPS-treated U937 cells and TNF-alpha-stimulated HUVECs by suppressing the activation of the NF-kappa B pathway. |
资助项目 | National Basic Research Program of China (973 Program)[2007CB935804] ; National Science Funds Committee[30500612] ; National Science Funds Committee[30570565] ; National Science Funds Committee[C03020706] |
WOS关键词 | NF-KAPPA-B ; HUMAN ENDOTHELIAL-CELLS ; ADENOSINE A(1) RECEPTOR ; SIGNAL-TRANSDUCTION ; EPITHELIAL-CELLS ; UP-REGULATION ; INFLAMMATORY REACTIONS ; GASTRIC-CANCER ; TNF-ALPHA ; IN-VITRO |
WOS研究方向 | Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000294367700025 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/278423] |
专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
通讯作者 | Feng, Linyin |
作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Jin, Lei,Zhang, Li-Min,Xie, Ke-Qiang,et al. Paeoniflorin suppresses the expression of intercellular adhesion molecule-1 (ICAM-1) in endotoxin-treated human monocytic cells[J]. BRITISH JOURNAL OF PHARMACOLOGY,2011,164(2B):694-703. |
APA | Jin, Lei,Zhang, Li-Min,Xie, Ke-Qiang,Ye, Yang,&Feng, Linyin.(2011).Paeoniflorin suppresses the expression of intercellular adhesion molecule-1 (ICAM-1) in endotoxin-treated human monocytic cells.BRITISH JOURNAL OF PHARMACOLOGY,164(2B),694-703. |
MLA | Jin, Lei,et al."Paeoniflorin suppresses the expression of intercellular adhesion molecule-1 (ICAM-1) in endotoxin-treated human monocytic cells".BRITISH JOURNAL OF PHARMACOLOGY 164.2B(2011):694-703. |
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