Potentiation of the Cytotoxic Activity of Copper by Polyphosphate on Biofilm-Producing Bacteria: A Bioinspired Approach

doi:10.3390/md10112369

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	Potentiation of the Cytotoxic Activity of Copper by Polyphosphate on Biofilm-Producing Bacteria: A Bioinspired Approach
	Mueller, Werner E. G.2; Wang, Xiaohong 1,2; Guo, Yue-Wei3 ; Schroeder, Heinz C.2,3
刊名	MARINE DRUGS
	2012-11
卷号	10 期号:11 页码:2369-2387
关键词	biofilm marine coatings antifouling strategies synergism polyphosphate copper bisphosphonate bioinspired approach
ISSN号	1660-3397
DOI	10.3390/md10112369
文献子类	Article
英文摘要	Adhesion and accumulation of organic molecules represent an ecologically and economically massive problem. Adhesion of organic molecules is followed by microorganisms, unicellular organisms and plants together with their secreted soluble and structure-associated byproducts, which damage unprotected surfaces of submerged marine structures, including ship hulls and heat exchangers of power plants. This is termed biofouling. The search for less toxic anti-biofilm strategies has intensified since the ban of efficient and cost-effective anti-fouling paints, enriched with the organotin compound tributyltin, not least because of our finding of the ubiquitous toxic/pro-apoptotic effects displayed by this compound [1]. Our proposed bio-inspired approach for controlling, suppressing and interfluencing the dynamic biofouling complex uses copper as one component in an alternative anti-fouling system. In order to avoid and overcome the potential resistance against copper acquired by microorganisms we are using the biopolymer polyphosphate (polyP) as a further component. Prior to being functionally active, polyP has to be hydrolyzed to ortho-phosphate which in turn can bind to copper and export the toxic compound out of the cell. It is shown here that inhibition of the hydrolysis of polyP by the bisphosphonate DMDP strongly increases the toxic effect of copper towards the biofilm-producing Streptococcus mutans in a synergistic manner. This bisphosphonate not only increases the copper-caused inhibition of cell growth but also of biofilm production by the bacteria. The defensin-related ASABF, a marine toxin produced by the sponge Suberites domuncula, caused only an additive inhibitory effect in combination with copper. We conclude that the new strategy, described here, has a superior anti-biofilm potential and can be considered as a novel principle for developing bio-inspired antifouling compounds, or cocktails of different compounds, in the future.
资助项目	ERC[268476 BIOSILICA] ; European Commission[PIAPP-GA-2011-286059-CoreShell] ; European Commission[PIRSES-GA-2009-246987-MarBioTec EU-CN] ; European-Chinese Research Staff Exchange Cluster on Marine Biotechnology[00000000] ; European Commission/EUREKA (EUROSTARS)[4289-SILIBACTS] ; Public Welfare Project of Ministry of Land and Resources of the People's Republic of China[201011005-06] ; International Science & Technology Cooperation Program of China[2008DFA00980]
WOS关键词	MR CONTRAST AGENTS ; INORGANIC POLYPHOSPHATE ; MULTIXENOBIOTIC RESISTANCE ; MARINE SPONGE ; SUBERITES-DOMUNCULA ; OXIDE ; IRON ; EXPRESSION ; SURFACES ; CELLS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000311425800001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277887]
专题	天然药物化学研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Mueller, Werner E. G.
作者单位	1.Chinese Acad Geol Sci, Natl Res Ctr Geoanal, CN-100037 Beijing, Peoples R China; 2.Johannes Gutenberg Univ Mainz, Univ Med Ctr, ERC Adv Investigator Grant Res Grp, Inst Physiol Chem, D-55128 Mainz, Germany; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, CN-201203 Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Mueller, Werner E. G.,Wang, Xiaohong,Guo, Yue-Wei,et al. Potentiation of the Cytotoxic Activity of Copper by Polyphosphate on Biofilm-Producing Bacteria: A Bioinspired Approach[J]. MARINE DRUGS,2012,10(11):2369-2387.
APA	Mueller, Werner E. G.,Wang, Xiaohong,Guo, Yue-Wei,&Schroeder, Heinz C..(2012).Potentiation of the Cytotoxic Activity of Copper by Polyphosphate on Biofilm-Producing Bacteria: A Bioinspired Approach.MARINE DRUGS,10(11),2369-2387.
MLA	Mueller, Werner E. G.,et al."Potentiation of the Cytotoxic Activity of Copper by Polyphosphate on Biofilm-Producing Bacteria: A Bioinspired Approach".MARINE DRUGS 10.11(2012):2369-2387.