Characterization of the conversion between CD133(+) and CD133(-) cells in colon cancer SW620 cell line

doi:10.4161/cbt.22000

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	Characterization of the conversion between CD133(+) and CD133(-) cells in colon cancer SW620 cell line
	Feng, Jian-Ming 1; Miao, Ze-Hong 1; Jiang, Yi1 ; Chen, Yi1 ; Li, Jia-Xin 1; Tong, Lin-Jiang 1; Zhang, Jin 2; Huang, Yi-Ran 2; Ding, Jian1
刊名	CANCER BIOLOGY & THERAPY
	2012-12
卷号	13 期号:14 页码:1396-1406
关键词	CD133 conversion cancer stem cells SW620 colon cancer
ISSN号	1538-4047
DOI	10.4161/cbt.22000
文献子类	Article
英文摘要	The state of cancer stem cells (CSC) under reversible fluctuations, which has been revealed in breast cancer cells most recently, suggests that subpopulations with distinct phenotypes and functions within cancer cells can undergo inter-conversion. To investigate the possibility in colon cancer cells, we employed CD133 as the CSC marker, and characterized CD133 expression pattern and the biological features of the CD133(+) and CD133(-) subsets. Flow cytometry revealed that CD133 was bimodally expressed in SW620 cells among eight colon cancer cell lines. The CD133(+) clonal SW620 cells displayed a differential gene expression profile, higher cellular reactive oxygen species (ROS), enhanced tumorigenesis and resistance to 5-fluorouracil. The conversion in term of the CD133 phenotype of the sorted cells was observed in vitro and in vivo. The fraction of the CD133(+) cells decreased from 99% to 80% in the sorted CD133(+) population while rising from 5% to 10% in the sorted CD133(-) population during the first 20-d cultivation and then stayed almost unchanged. A fraction (about 20%) of the CD133(+) clonal cells lost their CD133 marker while about 10% of the CD133(-) nclonal cells acquired the CD133 marker. 5-Azacytidine enhanced the fraction of the CD133(+) cells in both of the CD133(+) and CD133(-) clonal cells. Our data demonstrate that CD133 expression is dynamic and reversible, and reveal the inter-conversion between the CD133(+) and the CD133(-) SW620 cells, suggesting that the CD133 phenotype of SW620 cell population is retained by the conversion between the two cell subsets.
资助项目	National Natural Science Foundation of China[81025020] ; National Natural Science Foundation of China[81021062] ; National Basic Research Program of China[2012CB932502] ; National Science and Technology Major Project of China[2012ZX09301-001-002] ; "Interdisciplinary Cooperation Team" Program for Science and Technology Innovation of the Chinese Academy of Sciences[00000000] ; Science and Technology Commission of Shanghai Municipality (STCSM)[09540704100]
WOS关键词	STEM-CELLS ; INITIATING CELLS ; IDENTIFICATION ; POPULATIONS ; EXPRESSION ; MELANOMA ; PATHWAY ; GROWTH ; TUMORS ; STATE
WOS研究方向	Oncology
语种	英语
出版者	LANDES BIOSCIENCE
WOS记录号	WOS:000312212200007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277860]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Miao, Ze-Hong
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 200031, Peoples R China; 2.Shanghai Jiao Tong Univ, Dept Urol, Renji Hosp, Sch Med, Shanghai 200030, Peoples R China
推荐引用方式 GB/T 7714	Feng, Jian-Ming,Miao, Ze-Hong,Jiang, Yi,et al. Characterization of the conversion between CD133(+) and CD133(-) cells in colon cancer SW620 cell line[J]. CANCER BIOLOGY & THERAPY,2012,13(14):1396-1406.
APA	Feng, Jian-Ming.,Miao, Ze-Hong.,Jiang, Yi.,Chen, Yi.,Li, Jia-Xin.,...&Ding, Jian.(2012).Characterization of the conversion between CD133(+) and CD133(-) cells in colon cancer SW620 cell line.CANCER BIOLOGY & THERAPY,13(14),1396-1406.
MLA	Feng, Jian-Ming,et al."Characterization of the conversion between CD133(+) and CD133(-) cells in colon cancer SW620 cell line".CANCER BIOLOGY & THERAPY 13.14(2012):1396-1406.