Danthron activates AMP-activated protein kinase and regulates lipid and glucose metabolism in vitro

doi:10.1038/aps.2013.39

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 生物技术药物研发中心（筹）

	Danthron activates AMP-activated protein kinase and regulates lipid and glucose metabolism in vitro
	Zhou, Rong 1; Wang, Ling 1; Xu, Xing 2; Chen, Jing2 ; Hu, Li-hong 2; Chen, Li-li2 ; Shen, Xu1,2
刊名	ACTA PHARMACOLOGICA SINICA
	2013-08
卷号	34 期号:8 页码:1061-1069
关键词	1,8-dihydroxyanthraquinone (danthron) traditional Chinese medicine AMPK SREBP1c fatty acid synthetase cholesterol triglyceride glucose consumption
ISSN号	1671-4083
DOI	10.1038/aps.2013.39
文献子类	Article
英文摘要	Aim: To discover the active compound on AMP-activated protein kinase (AMPK) activation and investigate the effects of the active compound 1,8-dihydroxyanthraquinone (danthron) from the traditional Chinese medicine rhubarb on AMPK-mediated lipid and glucose metabolism in vitro. Methods: HepG2 and C2C12 cells were used. Cell viability was determined using MTT assay. Real-time PCR was performed to measure the gene expression. Western blotting assay was applied to investigate the protein phosphorylation level. Enzymatic assay kits were used to detect the total cholesterol (TC), triglyceride (TG) and glucose contents. Results: Danthron (0.1, 1, and 10 mu mol/L) dose-dependently promoted the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in both HepG2 and C2C12 cells. Meanwhile, danthron treatment significantly reduced the lipid synthesis related sterol regulatory element-binding protein 1c (SREBP1c) and fatty acid synthetase (FAS) gene expressions, and the TC and TG levels. In addition, danthron treatment efficiently increased glucose consumption. The actions of danthron on lipid and glucose metabolism were abolished or reversed by co-treatment with the AMPK inhibitor compound C. Conclusion: Danthron effectively reduces intracellular lipid contents and enhanced glucose consumption in vitro via activation of AMPK signaling pathway.
资助项目	National Science and Technology Major Project[2012ZX09301001-004] ; National Science and Technology Major Project[2012ZX09103101-18] ; National Natural Science Foundation of China[81173105] ; National Natural Science Foundation of China[91213306] ; Foundation of Chinese Academy of Sciences[KSCX2-EW-Q-3] ; National Marine 863 Programme[2011AA09070102] ; Science Foundation of Shanghai[11XD1406100]
WOS关键词	ENHANCES BASOLATERAL EFFLUX ; CARDIOVASCULAR-DISEASE ; RESPONSIVE ELEMENT ; GENE-EXPRESSION ; CACO-2 CELLS ; HEPG2 CELLS ; CHOLESTEROL ; LIVER ; PHOSPHORYLATION ; IDENTIFICATION
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:4896742
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000322709400010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277519]
专题	生物技术药物研发中心（筹）中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Chen, Li-li
作者单位	1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Rong,Wang, Ling,Xu, Xing,et al. Danthron activates AMP-activated protein kinase and regulates lipid and glucose metabolism in vitro[J]. ACTA PHARMACOLOGICA SINICA,2013,34(8):1061-1069.
APA	Zhou, Rong.,Wang, Ling.,Xu, Xing.,Chen, Jing.,Hu, Li-hong.,...&Shen, Xu.(2013).Danthron activates AMP-activated protein kinase and regulates lipid and glucose metabolism in vitro.ACTA PHARMACOLOGICA SINICA,34(8),1061-1069.
MLA	Zhou, Rong,et al."Danthron activates AMP-activated protein kinase and regulates lipid and glucose metabolism in vitro".ACTA PHARMACOLOGICA SINICA 34.8(2013):1061-1069.