Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes

doi:10.1007/s13238-013-0016-x

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 新药研究国家重点实验室

	Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes
	Gu, Ying 1; Liu, Guang-Hui 2,3; Plongthongkum, Nongluk 4; Benner, Christopher 1; Yi, Fei 1; Qu, Jing 2; Suzuki, Keiichiro 1; Yang, Jiping 2; Zhang, Weiqi 2; Li, Mo 1
刊名	PROTEIN & CELL
	2014-01
卷号	5 期号:1 页码:59-68
关键词	human cardiomyocyte DNA methylation microarray heart development
ISSN号	1674-800X
DOI	10.1007/s13238-013-0016-x
文献子类	Article
英文摘要	With defined culture protocol, human embryonic stem cells (hESCs) are able to generate cardiomyocytes in vitro, therefore providing a great model for human heart development, and holding great potential for cardiac disease therapies. In this study, we successfully generated a highly pure population of human cardiomyocytes (hCMs) (> 95% cTnT(+)) from hESC line, which enabled us to identify and characterize an hCM-specific signature, at both the gene expression and DNA methylation levels. Gene functional association network and gene-disease network analyses of these hCM-enriched genes provide new insights into the mechanisms of hCM transcriptional regulation, and stand as an informative and rich resource for investigating cardiac gene functions and disease mechanisms. Moreover, we show that cardiac-structural genes and cardiac-transcription factors have distinct epigenetic mechanisms to regulate their gene expression, providing a better understanding of how the epigenetic machinery coordinates to regulate gene expression in different cell types.
资助项目	National Basic Research Program (973 Program)[2014CB910500] ; Chinese Academy of Sciences[XDA01020312] ; National Basic Research Program of China (973 Program)[2014CB964600] ; National Natural Science Foundation[81271266] ; National Natural Science Foundation[31222039] ; National Laboratory of Biomacromolecules[00000000] ; State Key Laboratory of Drug Research[SIMM1302KF-17] ; CIRM[00000000] ; California Institute for Regenerative Medicine[RB3-05083] ; NIH[R01 GM097253] ; TERCEL-ISCIII-MINECO[00000000] ; CIBER-BBN[00000000] ; Cardiocel[00000000] ; Fundacion Cellex[00000000] ; G. Harold and Leila Y. Mathers Charitable Foundation[00000000] ; Leona M. and Harry B. Helmsley Charitable Trust[00000000] ; Ellison Medical Foundation[00000000]
WOS关键词	EMBRYONIC STEM-CELLS ; GENE REGULATORY NETWORKS ; CYTOSCAPE PLUGIN ; CARDIAC DIFFERENTIATION ; OPTIMIZATION ; SIGNATURE
WOS研究方向	Cell Biology
语种	英语
出版者	HIGHER EDUCATION PRESS
WOS记录号	WOS:000332461300008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277304]
专题	新药研究国家重点实验室中科院受体结构与功能重点实验室
通讯作者	Liu, Guang-Hui
作者单位	1.Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 USA; 2.Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State key Lab Drug Res, Shanghai 201203, Peoples R China; 4.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA; 5.Ctr Regenerat Med Barcelona, Barcelona 08003, Spain; 6.Biomed Res Networking Ctr Bioengn Biomat & Nanome, Sargossa 50018, Spain; 7.Univ Luxembourg, LCSB, Walferdange, Luxembourg
推荐引用方式 GB/T 7714	Gu, Ying,Liu, Guang-Hui,Plongthongkum, Nongluk,et al. Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes[J]. PROTEIN & CELL,2014,5(1):59-68.
APA	Gu, Ying.,Liu, Guang-Hui.,Plongthongkum, Nongluk.,Benner, Christopher.,Yi, Fei.,...&Izpisua Belmonte, Juan Carlos.(2014).Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes.PROTEIN & CELL,5(1),59-68.
MLA	Gu, Ying,et al."Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes".PROTEIN & CELL 5.1(2014):59-68.