Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry

doi:10.1016/j.aca.2014.02.019

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	Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry
	Yan, Zhixiang 1; Lin, Ge 2; Yec, Yang 3; Wang, Yitao 1; Yan, Ru 1
刊名	ANALYTICA CHIMICA ACTA
	2014-03-28
卷号	819 页码:56-64
关键词	Triterpenoid saponins profiling Adducts targeted neutral loss scanning Triple quadrupole linear ion trap mass spectrometry
ISSN号	0003-2670
DOI	10.1016/j.aca.2014.02.019
文献子类	Article
英文摘要	Triterpenoid saponins (TSs) are a unique class of high molecular weight glycosides and have been frequently used in cosmetic and phytotherapy industry. There is a great need to comprehensively profile these plant metabolites for studying their functions. In the present study, a novel adducts targeted neutral loss (NL), triggered enhanced resolution (ER) and enhanced product ion (EPI) scanning approach were described for TSs profiling using a triple quadrupole linear ion trap mass spectrometry. This approach circumvented the disadvantages of poor glycosidic bond cleavage of TSs by monitoring the NH3 (NL17) and HCOOH (NL46) loss of their abundant ammonium and formate adducts, respectively. The sugar-loss independent NL scanning served as a sensitive survey scan and triggered information-dependent ER and EPI scans to increase peak assignment confidence. NL17 was superior to NL46 for TSs characterization due to the better fragmentation of ammonium adducts than formate adducts. For those TSs undetectable by NL17, precursor ion (PI) scan for sapogenin fragments could be used to screen out non-adducted TSs. The NL/PI-ER-EPI approach was applied for TSs profiling in Astragali Radix, a famous medicinal and nutritional plant widely used in Asian countries and United States. In total, 136 TSs were detected while previous research using high resolution mass spectrometry based full scan only detected 22 TSs in this herb. (C) 2014 Elsevier B.V. All rights reserved.
资助项目	Science and Technology Development Fund of Macao SAR[043/2011/A2] ; Research Committee of University of Macau[MYRG207(Y2-L4)-ICMS11-YR]
WOS关键词	LIQUID-CHROMATOGRAPHY ; RADIX ASTRAGALI ; STRUCTURAL-CHARACTERIZATION ; ROOTS ; IDENTIFICATION ; FRAGMENTATION ; BIOSYNTHESIS ; GINSENOSIDES ; INFORMATION ; METABOLITES
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000332968500007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277149]
专题	新药研究国家重点实验室中科院受体结构与功能重点实验室
通讯作者	Yan, Ru
作者单位	1.Univ Macau, Inst Chinese Med Sci, Key Lab Qual Res Chinese Med, Taipa, Peoples R China; 2.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yan, Zhixiang,Lin, Ge,Yec, Yang,et al. Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2014,819:56-64.
APA	Yan, Zhixiang,Lin, Ge,Yec, Yang,Wang, Yitao,&Yan, Ru.(2014).Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry.ANALYTICA CHIMICA ACTA,819,56-64.
MLA	Yan, Zhixiang,et al."Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry".ANALYTICA CHIMICA ACTA 819(2014):56-64.