A Generic Multiple Reaction Monitoring Based Approach for Plant Flavonoids Profiling Using a Triple Quadrupole Linear Ion Trap Mass Spectrometry | |
Yan, Zhixiang1; Lin, Ge2; Ye, Yang3![]() | |
刊名 | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
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2014-06 | |
卷号 | 25期号:6页码:955-965 |
关键词 | Flavonoids profiling Multiple reaction monitoring Triple quadrupole linear ion trap mass spectrometry |
ISSN号 | 1044-0305 |
DOI | 10.1007/s13361-014-0863-6 |
文献子类 | Article |
英文摘要 | Flavonoids are one of the largest classes of plant secondary metabolites serving a variety of functions in plants and associating with a number of health benefits for humans. Typically, they are co-identified with many other secondary metabolites using untargeted metabolomics. The limited data quality of untargeted workflow calls for a shift from the breadth-first to the depth-first screening strategy when a specific biosynthetic pathway is focused on. Here we introduce a generic multiple reaction monitoring (MRM)-based approach for flavonoids profiling in plants using a hybrid triple quadrupole linear ion trap (QTrap) mass spectrometer. The approach includes four steps: (1) preliminary profiling of major aglycones by multiple ion monitoring triggered enhanced product ion scan (MIM-EPI); (2) glycones profiling by precursor ion triggered EPI scan (PI-EPI) of major aglycones; (3) comprehensive aglycones profiling by combining MIM-EPI and neutral loss triggered EPI scan (NL-EPI) of major glycone; (4) in-depth flavonoids profiling by MRM-EPI with elaborated MRM transitions. Particularly, incorporation of the NH3 loss and sugar elimination proved to be very informative and confirmative for flavonoids screening. This approach was applied for profiling flavonoids in Astragali radix (Huangqi), a famous herb widely used for medicinal and nutritional purposes in China. In total, 421 flavonoids were tentatively characterized, among which less than 40 have been previously reported in this medicinal plant. This MRM-based approach provides versatility and sensitivity that required for flavonoids profiling in plants and serves as a useful tool for plant metabolomics. |
资助项目 | Science and Technology Development Fund of Macao SAR[043/2011/A2] ; Research Committee of University of Macau[MYRG207(Y2-L4)-ICMS11-YR] |
WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY-IONIZATION ; NEUTRAL LOSS ; PHENOLIC-COMPOUNDS ; HUMAN URINE ; RED WINE ; METABOLITES ; MS/MS ; QUANTIFICATION ; IDENTIFICATION |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000336025800009 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/277058] ![]() |
专题 | 天然药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
通讯作者 | Yan, Ru |
作者单位 | 1.Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Taipa, Peoples R China; 2.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
推荐引用方式 GB/T 7714 | Yan, Zhixiang,Lin, Ge,Ye, Yang,et al. A Generic Multiple Reaction Monitoring Based Approach for Plant Flavonoids Profiling Using a Triple Quadrupole Linear Ion Trap Mass Spectrometry[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2014,25(6):955-965. |
APA | Yan, Zhixiang,Lin, Ge,Ye, Yang,Wang, Yitao,&Yan, Ru.(2014).A Generic Multiple Reaction Monitoring Based Approach for Plant Flavonoids Profiling Using a Triple Quadrupole Linear Ion Trap Mass Spectrometry.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,25(6),955-965. |
MLA | Yan, Zhixiang,et al."A Generic Multiple Reaction Monitoring Based Approach for Plant Flavonoids Profiling Using a Triple Quadrupole Linear Ion Trap Mass Spectrometry".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 25.6(2014):955-965. |
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