Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea

doi:10.1039/c5ra04327a

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea
	Qiao, Xue 1; Song, Wei 1; Wang, Qi 1; Liu, Ke-di 1; Zhang, Zheng-xiang 3; Bo, Tao 3; Li, Ren-yong 4; Liang, Li-na 4; Tzeng, Yew-min 5; Guo, De-an1,2
刊名	RSC ADVANCES
	2015
卷号	5 期号:58 页码:47040-47052
ISSN号	2046-2069
DOI	10.1039/c5ra04327a
文献子类	Article
英文摘要	Antrodia cinnamomea is a precious medicinal mushroom used in adjuvant treatments of cancer. Triterpenoids (25R/S-ergostane epimers and Delta(7,9)/Delta(8)-lanostanes) and polysaccharides are its major bioactive constituents. Quality control of this mushroom is difficult, due to a lack of effective analytical methods. In this study, a series of methods were established to analyze A. cinnamomea. The ergostane- and lanostane-type triterpenoids were extracted by 50% and 100% methanol, respectively, and were then analyzed by ultra-high performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (UHPLC/DAD/qTOF-MS). In total 49 compounds, including 34 ergostanes and 10 lanostanes, were characterized by comparing with 31 reference standards or by analyzing the UV and MS spectral data. Furthermore, the contents of 18 major triterpenoids, including 10 ergostanes and 8 lanostanes, were determined by UPLC/UV or supercritical fluid chromatography coupled with mass spectrometry (SFC/MS, for 25R/S-antcin A) within 16 min. This is the first time that the contents of pure optical forms for 5 pairs of 25R- and 25S-ergostane epimers (antcins A, B, C, H, and K) were determined. On the other hand, the polysaccharides in A. cinnamomea were analyzed by ion chromatography coupled with pulsed amperometric detection (IC/PAD) after acid hydrolysis, and the contents of 7 monosaccharides were determined. Finally, the established methods were applied to the analysis of 15 batches of A. cinnamomea. Samples derived from different cultivation techniques could be distinguished according to the contents of triterpenoids. The UPLC/UV, SFC/MS and IC/PAD methods established in this work provided powerful tools to evaluate the quality of A. cinnamomea.
资助项目	National Natural Science Foundation of China[81222054] ; National Natural Science Foundation of China[81303294] ; Program for New Century Excellent Talents in University from Chinese Ministry of Education[NCET-11-0019] ; State Key Laboratory of Drug Research[SIMM1403KF-16]
WOS关键词	ANION-EXCHANGE CHROMATOGRAPHY ; PULSED AMPEROMETRIC DETECTION ; MASS-SPECTROMETRY ; GANODERMA-LUCIDUM ; FRUITING BODIES ; CAMPHORATA ; ERGOSTANE ; SEPARATION ; ACID
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000355702900075
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276728]
专题	上海中药现代化研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Ye, Min
作者单位	1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Agilent Technol, Beijing 100102, Peoples R China; 4.ThermoFisher Sci Ltd, Beijing 100085, Peoples R China; 5.Chaoyang Univ Technol, Inst Biochem Sci & Technol, Taichung 41349, Taiwan
推荐引用方式 GB/T 7714	Qiao, Xue,Song, Wei,Wang, Qi,et al. Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea[J]. RSC ADVANCES,2015,5(58):47040-47052.
APA	Qiao, Xue.,Song, Wei.,Wang, Qi.,Liu, Ke-di.,Zhang, Zheng-xiang.,...&Ye, Min.(2015).Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea.RSC ADVANCES,5(58),47040-47052.
MLA	Qiao, Xue,et al."Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea".RSC ADVANCES 5.58(2015):47040-47052.