Magnetic solid-phase extraction coupled with HPLC-Q-TOF-MS for rapid analysis of tyrosinase binders from San-Bai decoction by Box-Behnken statistical design | |
Tao, Yi2,3; Su, Dandan2,3; Du, Yingshan2,3; Li, Weidong2,3; Cai, Baochang2,3; Di, Liuqing2; Shi, Liyun1; Hu, Lihong4 | |
刊名 | RSC ADVANCES
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2016 | |
卷号 | 6期号:111页码:109730-109741 |
ISSN号 | 2046-2069 |
DOI | 10.1039/c6ra22045b |
文献子类 | Article |
英文摘要 | Tyrosinase is the rate-limiting enzyme for controlling the production of melanin. A very precise, sensitive and convenient solid-phase extraction based approach was developed for extract and analysis of tyrosinase binders from San-Bai decoction. Tyrosinase binders were captured by using tyrosinase conjugated magnetic beads. The retained tyrosinase binders were dissociated and analyzed by high performance liquid chromatography and quadrupole-time-of-flight mass spectrometry. The powder X-ray diffraction (XRD), transmission electron microscopy (TEM), vibration sample magnetometer (VSM) and Fourier transform infrared (FT-IR) spectroscopy techniques were used for the characterization of tyrosinase conjugated magnetic beads. A variety of experimental conditions such as incubation temperature, buffer pH and ion strength, which may affect extraction yield, were optimized through Box-Behnken statistical design and response surface methodology by using paeoniflorin as a model compound. The optimal incubation conditions were as follows: wash times: 4, wash solvent: 10% acetonitrile-water, incubation time: 30 min, temperature: 34.84 degrees C, ion strength: 193.67 mM, pH: 6.98. The established approach was successfully applied for the extraction of tyrosinase binders from San-Bai decoction. The tyrosinase inhibitory assay was employed for validation of the activities of these identified binders. Fifteen tyrosinase inhibitors were identified, and nine of which, including benzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, paeonolide, oxypaeoniflora, albiflorin, ononin, mudanpioside C and apiopaeonoside, were reported for the first time. |
资助项目 | Natural Science Foundation for the Youth of Jiangsu Province[BK20140963] ; Nutritional Science Foundation of By-Health Co.[TY0141103] ; Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)[00000000] |
WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; FLIGHT-MASS-SPECTROMETRY ; CHINESE MEDICINE FORMULAS ; DIODE-ARRAY ; INHIBITORS ; ROOT ; QUANTIFICATION ; IDENTIFICATION ; LICORICE ; STRATEGY |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000389350700045 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/276186] ![]() |
专题 | 上海中药现代化研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
通讯作者 | Tao, Yi |
作者单位 | 1.Nanjing Univ Chinese Med, Dept Microbiol & Immunol, Nanjing 210023, Jiangsu, Peoples R China; 2.Nanjing Univ Chinese Med, Sch Pharm, Xianlin Campus,138 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China; 3.Nanjing Univ Chinese Med, Jiangsu Key Lab Chinese Med Proc, Nanjing 210023, Jiangsu, Peoples R China; 4.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Tao, Yi,Su, Dandan,Du, Yingshan,et al. Magnetic solid-phase extraction coupled with HPLC-Q-TOF-MS for rapid analysis of tyrosinase binders from San-Bai decoction by Box-Behnken statistical design[J]. RSC ADVANCES,2016,6(111):109730-109741. |
APA | Tao, Yi.,Su, Dandan.,Du, Yingshan.,Li, Weidong.,Cai, Baochang.,...&Hu, Lihong.(2016).Magnetic solid-phase extraction coupled with HPLC-Q-TOF-MS for rapid analysis of tyrosinase binders from San-Bai decoction by Box-Behnken statistical design.RSC ADVANCES,6(111),109730-109741. |
MLA | Tao, Yi,et al."Magnetic solid-phase extraction coupled with HPLC-Q-TOF-MS for rapid analysis of tyrosinase binders from San-Bai decoction by Box-Behnken statistical design".RSC ADVANCES 6.111(2016):109730-109741. |
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