Comparative pharmacokinetic analysis of extracts of crude and wine-processed Dipsacus asper in rats by a sensitive ultra performance liquid chromatography-tandem mass spectrometry approach

doi:10.1016/j.jchromb.2016.09.024

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	Comparative pharmacokinetic analysis of extracts of crude and wine-processed Dipsacus asper in rats by a sensitive ultra performance liquid chromatography-tandem mass spectrometry approach
	Tao, Yi 1,2; Ren, Yuchao 1,2; Li, Weidong 1,2; Cai, Baochang 1,2; Di, Liuqing 1; Shi, Liyun 3; Hu, Lihong 4
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2016-11-15
卷号	1036 页码:33-41
关键词	Dipsacus asper Wine-processed UPLC-MS/MS Pharmacokinetics
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2016.09.024
文献子类	Article
英文摘要	The purpose of this study is to establish and validate an UPLC MS/MS approach to determine 4-caffeoylquinic acid, chlorogenic acid, 3,5-dicaffeoylquinic acid, loganic acid, loganin, sweroside, dipsacoside B and asperosaponin VI from extracts of crude and wine-processed Dipsacus asper in biological samples and apply the approach to a comparative pharmacokinetic study. A Waters BEH C-18 UPLC column was employed with acetonitrile/0.2% formic acid-water as mobile phases. The mass analysis was carried out in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan made. A one-step protein precipitation by acetonitrile was performed to extract the eight analytes from plasma. Our results revealed that all of the calibration curves displayed good linear regression (r(2) > 0.9990). The lower limits of quantification (LLOQ) were determined as 10.0, 9.6, 8.9, 9.1, 9.2, 9.8,10.1 and 9.8 ng/mL. The intra-day and inter-day precisions (RSD) of the eight compounds at high, medium and low levels were less than 4.94% and the bias of the accuracies ranged from -3.89% to 3.95%.The extraction recoveries of the eight compounds were from 90.4% to 100.2% and the matrix effects ranged from 89.3% to 100.1%. The stabilities of these compounds were investigated by analyzing six replicates of QC samples at three different concentrations following storage at 25 degrees C for 4 h, 80 degrees C for 30 days, three freeze -thaw cycles, and 4 degrees C for 24 h. All the samples showed satisfactory precision and accuracy after various stability tests. Pharmacokinetic parameters were estimated using a non-compartment model. Compared with the crude group, the parameters of C-max and AUC(0-t) of 4-caffeoylquinic acid, loganic acid, loganin and asperosaponin VI increased remarkably (p<0.05) after oral administration 'of the aqueous extract of wine-processed Dipsacus asper, indicating that wine-processing could enhance bioavailability of 4-caffeoylquinic acid, loganic acid, loganin and asperosaponin VI. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	Nutritional Science Foundation of By-Health Co.[TY0141103] ; Natural Science Foundation for the Youth of Jiangsu Province[BK20140963] ; Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)[00000000]
WOS关键词	ASPEROSAPONIN VI ; PLASMA ; PATHWAY ; CELLS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000387193800004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275811]
专题	上海中药现代化研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Tao, Yi
作者单位	1.Nanjing Univ Chinese Med, Sch Pharm, Xianlin Campus,138 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China; 2.Nanjing Univ Chinese Med, Jiangsu Key Lab Chinese Med Proc, Nanjing 210023, Jiangsu, Peoples R China; 3.Nanjing Univ Chinese Med, Dept Microbiol & Immunol, Nanjing 210023, Jiangsu, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Univ Chinese Acad Sci, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Tao, Yi,Ren, Yuchao,Li, Weidong,et al. Comparative pharmacokinetic analysis of extracts of crude and wine-processed Dipsacus asper in rats by a sensitive ultra performance liquid chromatography-tandem mass spectrometry approach[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1036:33-41.
APA	Tao, Yi.,Ren, Yuchao.,Li, Weidong.,Cai, Baochang.,Di, Liuqing.,...&Hu, Lihong.(2016).Comparative pharmacokinetic analysis of extracts of crude and wine-processed Dipsacus asper in rats by a sensitive ultra performance liquid chromatography-tandem mass spectrometry approach.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1036,33-41.
MLA	Tao, Yi,et al."Comparative pharmacokinetic analysis of extracts of crude and wine-processed Dipsacus asper in rats by a sensitive ultra performance liquid chromatography-tandem mass spectrometry approach".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1036(2016):33-41.