Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats

doi:10.1016/S0014-2999(00)00826-8

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	Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats
	Leng, Y; Gu, ZP ; Cao, L
刊名	EUROPEAN JOURNAL OF PHARMACOLOGY
	2000-12-08
卷号	409 期号:2 页码:123-131
关键词	droloxifene luteal cell apoptosis c-myc bax bcl-2 mRNA
ISSN号	0014-2999
DOI	10.1016/S0014-2999(00)00826-8
文献子类	Article
英文摘要	Droloxifene is a tamoxifen derivative whose effects in the therapy of human breast cancer and postmenopausal osteoporosis have been studied widely. We had found that droloxifene could induce apoptosis of luteal cells of rat in vitro, but its mechanisms were unknown. In the present study, the expression of c-myc, bax and bcl-2 mRNA in cultured rat luteal cells during apoptosis induced by droloxifene was investigated and possible associations between these genes and apoptosis were analyzed. Cultured luteal cells of rats were incubated with droloxifene at various concentrations and with treatment durations. Occurrence of apoptosis was detected by terminal deoxyribonucleotidyl tranferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL), DNA staining and DNA electrophoresis. Expression of these genes' mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the c-myc and bax mRNA levels increased as concentrations or treatment durations of droloxifene increased, while the bcl-2 mRNA level exhibited no changes. A marked increase of c-myc and bax mRNA appeared respectively with 12 and 24 h of treatment, while a clear increase of apoptosis of luteal cells was found at 18 h. These results suggested that droloxifene could induce apoptosis of luteal cells of rat in vitro. The increase of c-myc mRNA expression might be one of the initiating factors and the elevated ratio of bax/bcl-2 mRNA was also probably involved in this effect. (C) 2000 Elsevier Science B.V. All rights reserved.
WOS关键词	MESSENGER-RIBONUCLEIC-ACID ; RABBIT CORPUS-LUTEUM ; CORPORA-LUTEA ; HUMAN OVARY ; IN-VIVO ; FOLLICULAR-GROWTH ; GRANULOSA-CELLS ; DEATH ; REGRESSION ; LUTEOLYSIS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000165774000004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274546]
专题	新药研究国家重点实验室中科院受体结构与功能重点实验室
通讯作者	Gu, ZP
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.State Key Lab Drug Res, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Leng, Y,Gu, ZP,Cao, L. Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2000,409(2):123-131.
APA	Leng, Y,Gu, ZP,&Cao, L.(2000).Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats.EUROPEAN JOURNAL OF PHARMACOLOGY,409(2),123-131.
MLA	Leng, Y,et al."Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats".EUROPEAN JOURNAL OF PHARMACOLOGY 409.2(2000):123-131.