Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro | |
Wu, SF; Sun, HZ; Tu, ZH; Wu, HY | |
刊名 | ACTA PHARMACOLOGICA SINICA |
2001-03 | |
卷号 | 22期号:3页码:257-263 |
关键词 | epristeride prostate epithelium cultured cells epidermal growth factor receptors IGF type I receptor reverse polymerase chain reaction flow cytometry |
ISSN号 | 0253-9756 |
文献子类 | Article |
英文摘要 | AIM: To study the molecular mechanism of rat prostate atrophy induced by epristeride. METHODS: MTT test was used to determine the effect of epristeride on the growth of prostatic epithelial cell induced by exogenous epithelial growth factor (EGF) or insulin-like growth factor-I (IGF-I). RT-PCR and flow cytometry were then used to quantitatively detect the mRNA and protein expressions of EGFR and IGF-I R of the epithelial cells treated or untreated with epristeride. RESULTS: Epristeride attenuated growth of epithelial cells induced by exogenous EGF, IGF-I. Epristeride 360 nmol/L inhibited EGFR and IGF-I R expression at mRNA level, while epristeride 180 nmol/L had no marked effect on EGFR and IGF-I R mRNA expression. Both epristeride 180 nmol/L and 360 nmol/L could down regulate EGFR and IGF-IR protein levels. CONCLUSION: The molecular mechanisms of prostatic epithelial cell atrophy induced by epristeride might be associated with alteration in the expression of growth factor receptors such as EGF and IGF-I. |
WOS关键词 | IGF-BINDING PROTEINS ; FACTOR-I ; VENTRAL PROSTATE ; FACTOR-ALPHA ; 5-ALPHA-REDUCTASE ; FINASTERIDE ; RECEPTOR ; TISSUE ; LOCALIZATION ; HYPERPLASIA |
WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | ACTA PHARMACOLOGICA SINICA |
WOS记录号 | WOS:000167403800011 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/274513] |
专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
通讯作者 | Tu, ZH |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, State Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Fudan Univ, Med Ctr, Shanghai Peoples Hosp 1, Dept Urol, Shanghai 200032, Peoples R China |
推荐引用方式 GB/T 7714 | Wu, SF,Sun, HZ,Tu, ZH,et al. Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro[J]. ACTA PHARMACOLOGICA SINICA,2001,22(3):257-263. |
APA | Wu, SF,Sun, HZ,Tu, ZH,&Wu, HY.(2001).Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro.ACTA PHARMACOLOGICA SINICA,22(3),257-263. |
MLA | Wu, SF,et al."Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro".ACTA PHARMACOLOGICA SINICA 22.3(2001):257-263. |
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