Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity

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	Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity
	Qing, C ; Miao, ZH ; Tong, LJ ; Zhang, JS ; Ding, J
刊名	ACTA PHARMACOLOGICA SINICA
	2003-05
卷号	24 期号:5 页码:415-421
关键词	salvicine actinomycin D K562 cells cytotoxicity apoptosis
ISSN号	1671-4083
文献子类	Article
英文摘要	Aim: To study the effects of actinomycin D (Act D) on the cytotoxicity and apoptosis elicited by salvicine in human leukemia K562 cells. Methods: Growth inhibition of K562 cells was measured by the microculture tetrozolium (MTT) assay. Cell apoptosis was evaluated by fluorescence microscopy, DNA agarose gel electrophoresis, and flow cytometry. Results: Following exposure of K-562 cells to salvicine plus Act D for 24 h, Act D at the concentrations of 0.04, 0.4, and 4 mumol/L potentiated the cytotoxicity of salvicine 6.25 mumol/L to, some degree. The mean growth inhibitory rates went from 8% up to 69%, 71%, and 70%, respectively. However, the same enhancement of Act D did not continue to emerge at the higher concentrations than salvicine 6.25 mumol/L. Act D enhanced, or at least, did not decrease the cytotoxicity of salvicine against K562 cells. Fluorescence microscopy, DNA agarose gel electrophoresis, and flow cytometry revealed that Act D concentration-dependently inhibited the induction of apoptosis by salvicine in the same cell line. Conclusion: The combination of salvicine and Act D in a proper range of concentrations is able to enhance the cytotoxicity of salvicine against K562 cells though inhibiting apoptosis. The other mechanisms of cell death except apoptosis may be implicated in the process.
WOS关键词	MEDIATED APOPTOSIS ; CANCER CELLS ; DNA ; INDUCTION ; EXPRESSION ; CARCINOMA ; SURVIVAL ; MYELOMA ; AGENTS ; ASSAY
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:510065
出版者	SHANGHAI INST MATERIA MEDICA
WOS记录号	WOS:000182553500007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274236]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Ding, J
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Div Antitumor Ppharmacol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Dept Phytochem, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Qing, C,Miao, ZH,Tong, LJ,et al. Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity[J]. ACTA PHARMACOLOGICA SINICA,2003,24(5):415-421.
APA	Qing, C,Miao, ZH,Tong, LJ,Zhang, JS,&Ding, J.(2003).Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity.ACTA PHARMACOLOGICA SINICA,24(5),415-421.
MLA	Qing, C,et al."Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity".ACTA PHARMACOLOGICA SINICA 24.5(2003):415-421.