Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones

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	Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones
	Zhang, T; Li, JL; Xin, J; Ma, XC; Tu, ZH
刊名	ACTA PHARMACOLOGICA SINICA
	2004-02
卷号	25 期号:2 页码:171-175
关键词	fluoroquinolones phototoxic dermatitis comet assay
ISSN号	1671-4083
文献子类	Article
英文摘要	AIM: To compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones (FQ). METHODS: Lomefloxacin (LFLX), sparfloxacin (SPFX), ciprofloxacin (CPFX), and levofloxacin (LELX) were tested by comet assay and photodynamic DNA strand breaking activity under the different conditions of UVA irradiation. RESULTS: In comet assay, photogenotoxicity was evident at SPFX 1 mg/L, LFLX 5 mg/L, and CPFX 5 mg/L, and LELX 10 mg/L. In photodynamic DNA srand-breaking activity, SPFX and LFLX induced the conversion of the supercoiled form into the nicked relaxed form at 10-50 mumol/L, while CPFX at 25 mumol/L and LELX at 50 mumol/L. CONCLUSION: There were good correlations between the two methods to detect DNA damage induced by phototoxicity of fluoroquinolones. Photodynamic DNA strand breaking activity was a good method to detect DNA damage induced by photogenotoxicity of fluoroquinolones as well as comet assay.
WOS关键词	IN-VITRO ; COMET ASSAY ; PHOTOTOXICITY ; ANTIBACTERIAL ; TESTS ; CELLS
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	中文
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000188877300007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274115]
专题	药物安全性评价中心中科院受体结构与功能重点实验室新药研究国家重点实验室药理学第三研究室
通讯作者	Tu, ZH
作者单位	Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhang, T,Li, JL,Xin, J,et al. Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones[J]. ACTA PHARMACOLOGICA SINICA,2004,25(2):171-175.
APA	Zhang, T,Li, JL,Xin, J,Ma, XC,&Tu, ZH.(2004).Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones.ACTA PHARMACOLOGICA SINICA,25(2),171-175.
MLA	Zhang, T,et al."Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones".ACTA PHARMACOLOGICA SINICA 25.2(2004):171-175.