Quantitative characterization of 15-deoxy-Delta(12,14)-prostaglandin J(2) in regulating EGFP-Smad2 translocation in CHO cells through PPAR gamma/TGF beta/Smad2 pathway

doi:10.1159/000095183

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	Quantitative characterization of 15-deoxy-Delta(12,14)-prostaglandin J(2) in regulating EGFP-Smad2 translocation in CHO cells through PPAR gamma/TGF beta/Smad2 pathway
	Ye, Fei ; Sun, Tao ; Luo, Haibin ; Ding, Hong; Chen, Kaixian; Shen, Xu; Jiang, Hualiang
刊名	CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
	2006
卷号	18 期号:1-3 页码:143-150
关键词	15d-PGJ(2) EGFP-Smad2 translocation IN cell analyzer fluorescence rosiglitazone
ISSN号	1015-8987
DOI	10.1159/000095183
文献子类	Article
英文摘要	Smad2 is an important factor in TGF beta/Smad2 signal transduction pathway with ability for signal propagation, it could translocate from cytoplasm to nucleus after the TGF beta receptor-mediated phosphorylation. 15-deoxy-delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), a natural agonist of the peroxisome proliferator-activated receptor gamma (PPAR gamma) is found recently to be able to function in the regulation of Smad2 activity. However, no quantification data have been yet reported, and it still keeps suspenseful whether or not 15d-PGJ(2) could regulate Smad2 activity by depending on PPAR gamma through PPAR gamma/TGF beta/Smad2 pathway. In this work, by analyzing the EGFP-Smad2 location in CHO cells according to the Nucleus Trafficking Analysis Module based on IN Cell Analyzer 1000 platform, TGF beta stimulated EGFP-Smad2 translocation regulated by 15d-PGJ(2) was quantitatively investigated. The results showed that TGF beta could induce EGFP-Smad2 translocation from cytoplasm to nucleus by EC50 of 8.83 pM, and 15d-PGJ(2) could impede the TGF beta-stimulated Smad2 translocation by IC50 of 0.68 mu M. Moreover, GW9662, a PPAR gamma antagonist, could attenuate such a 15d-PGJ(2) inhibitory activity by almost one order of magnitude. This result thereby implies that 15d-PGJ(2) might inhibit Smad2 translocation through PPAR7/TGFP/Smad2 pathway. Further investigation discovered that different from the case for 15d-PGJ(2)' rosiglitazone, another PPAR gamma agonist, could enhance Smad2 translocation to nucleus, suggesting that rosiglitazone and 15d-PGJ(2) might take different modes in the activation of PPAR gamma within the signaling pathway. Copyright. (c) 2006 S. Karger AG, Basel.
WOS关键词	ACTIVATED-RECEPTOR-GAMMA ; TGF-BETA SUPERFAMILY ; PPAR-GAMMA ; GENE-EXPRESSION ; PROSTAGLANDIN J(2) ; SMAD PROTEINS ; DIFFERENTIATION ; MECHANISM ; MEMBRANE ; NUCLEUS
WOS研究方向	Cell Biology ; Physiology
语种	英语
出版者	KARGER
WOS记录号	WOS:000240629000015
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273699]
专题	药理学第三研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Shen, Xu
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
推荐引用方式 GB/T 7714	Ye, Fei,Sun, Tao,Luo, Haibin,et al. Quantitative characterization of 15-deoxy-Delta(12,14)-prostaglandin J(2) in regulating EGFP-Smad2 translocation in CHO cells through PPAR gamma/TGF beta/Smad2 pathway[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2006,18(1-3):143-150.
APA	Ye, Fei.,Sun, Tao.,Luo, Haibin.,Ding, Hong.,Chen, Kaixian.,...&Jiang, Hualiang.(2006).Quantitative characterization of 15-deoxy-Delta(12,14)-prostaglandin J(2) in regulating EGFP-Smad2 translocation in CHO cells through PPAR gamma/TGF beta/Smad2 pathway.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,18(1-3),143-150.
MLA	Ye, Fei,et al."Quantitative characterization of 15-deoxy-Delta(12,14)-prostaglandin J(2) in regulating EGFP-Smad2 translocation in CHO cells through PPAR gamma/TGF beta/Smad2 pathway".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 18.1-3(2006):143-150.