Differential expression of inducible nitric oxide synthase and IL-12 between peritoneal and splenic macrophages stimulated with LPS plus IFN-gamma is associated with the activation of extracellular signal-related kinase

doi:10.1093/intimm/dxl034

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	Differential expression of inducible nitric oxide synthase and IL-12 between peritoneal and splenic macrophages stimulated with LPS plus IFN-gamma is associated with the activation of extracellular signal-related kinase
	Zhu, YN ; Yang, YF ; Ono, S ; Zhong, XG ; Feng, YH ; Ren, YX ; Ni, J ; Fu, YF ; Tang, W; Zuo, JP
刊名	INTERNATIONAL IMMUNOLOGY
	2006-06
卷号	18 期号:6 页码:981-990
关键词	ERK iNOS IL-12 macrophage
ISSN号	0953-8178
DOI	10.1093/intimm/dxl034
文献子类	Article
英文摘要	Resident peritoneal macrophages (pM phi) are found deficient in T cell-stimulating capacity compared with the competent splenic macrophages (sM phi). Macrophages (M phi)-derived nitric oxide (NO) and IL-12 have been shown to play crucial roles in the interaction between M) and T cells. To further understand differential functions between pM phi and sM phi, we focused on the production of NO and IL-12 from LIPS plus IFN-gamma-activated M phi. We demonstrated the differential expression of inducible nitric oxide synthase (iNOS) and IL-12 in pM phi and sM phi with LIPS plus IFN-gamma stimulation. pM phi produced high level of NO but low level of IL-12, whereas sM phi produced high level of IL-12 but no NO. Furthermore, we demonstrated that there were no differences in IFN-gamma-induced signal transducer and activator of transcription-1 activation and consequent interferon regulatory factor-1 and interferon consensus sequence-binding protein up-regulation between pM phi and sM phi. Likewise, p38 mitogen-activated protein kinase was activated by LIPS with identical kinetics in both pM phi and sM phi. However, LIPS-induced extracellular signal-regulated kinase (Ill activation was prolonged in pM phi comparing with sM phi. Moreover, we demonstrated, using inhibitor selective for ERK cascade (111311398059), that the prolonged ERK activation contributed a positive signal for iNOS expression and a negative signal for IL-12p40 expression in resident pM phi. In addition, anti-IL-10-neutralizing antibody plus indomethacin could abrogate the inhibitory effects of endogenous IL-10 and prostaglandin E-2 on the production of IL-12 by resident pM phi possibly through suppressing ERK activation. Taken together, profound difference in ERK activation may account for differential LIPS plus IFN-gamma responsiveness between pM phi and sM phi. High production of NO and low production of IL-12 by pM phi may contribute to its deficiency in T cell-stimulating capacity.
WOS关键词	SEQUENCE-BINDING-PROTEIN ; TRANSCRIPTION FACTOR IRF-1 ; T-CELL PROLIFERATION ; REGULATORY FACTOR-I ; P40 GENE PROMOTER ; INTERLEUKIN-12 P40 ; INTERFERON-GAMMA ; LEISHMANIA-TROPICA ; DENDRITIC CELLS ; INDUCED INOS
WOS研究方向	Immunology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000238769400016
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273577]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Zuo, JP
作者单位	1.Chinese Acad Sci, Lab Immunopharmacol, Grad Sch,Shanghai Inst Biol Sci, State Key Lab Drug Res,Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Osaka Univ, Grad Sch Med, Dept Oncol, Suita, Osaka 5650871, Japan
推荐引用方式 GB/T 7714	Zhu, YN,Yang, YF,Ono, S,et al. Differential expression of inducible nitric oxide synthase and IL-12 between peritoneal and splenic macrophages stimulated with LPS plus IFN-gamma is associated with the activation of extracellular signal-related kinase[J]. INTERNATIONAL IMMUNOLOGY,2006,18(6):981-990.
APA	Zhu, YN.,Yang, YF.,Ono, S.,Zhong, XG.,Feng, YH.,...&Zuo, JP.(2006).Differential expression of inducible nitric oxide synthase and IL-12 between peritoneal and splenic macrophages stimulated with LPS plus IFN-gamma is associated with the activation of extracellular signal-related kinase.INTERNATIONAL IMMUNOLOGY,18(6),981-990.
MLA	Zhu, YN,et al."Differential expression of inducible nitric oxide synthase and IL-12 between peritoneal and splenic macrophages stimulated with LPS plus IFN-gamma is associated with the activation of extracellular signal-related kinase".INTERNATIONAL IMMUNOLOGY 18.6(2006):981-990.